用途
sufficient for 500 tests
品質等級
包裝
pkg of 1 96-well plate(s)
製造商／商標名
Calbiochem®
儲存條件
OK to freeze
avoid repeated freeze/thaw cycles
protect from light
輸入
sample type cultured cells (1,000-50,000 cells)
檢測方法
colorimetric
運輸包裝
wet ice
儲存溫度
−20°C
一般說明
Assay measures the increased activity of cellular mitochondrial dehydrogenases that can cleave the tetrazolium dye WST-1 to formazan. The formazan formation is then quantified by measuring the change in absorbance at 450 nm in a microplate reader. The activity of mitochondrial dehydrogenases is proportional to cell number. No washing, harvesting, or solubilization steps are required.
生化／生理作用
The Calbiochem Rapid Cell Proliferation Kit is designed to determine the number of viable cells in proliferation, or cytotoxicity studies, based on the cleavage of the tetrazolium salt WST-1 by mitochondrial dehydrogenases in viable cells. The assay can be used to measure cell proliferation in response to growth factors or cytokines, or to assess cytotoxic compounds.
特點和優勢
Assay Time: 0.5 - 4 h
Sensitivity: 1000 cells (adherent); 2500 cells (nonadherent)
準備報告
• WST-1 Labeling Mixture: Thaw the EMS solution at room temperature or in a 37°C water bath. Add the entire contents of this solution to the bottle containing the WST-1 reagent. The mixed solution can be stored at 4°C for several weeks. For long-term storage (several months) aliquot and store at -20°C. Protect from light.
其他說明
Ishiyama, M., et al. 1995. In Vitro Toxicology8, 187.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
Liu, S.O., et al 1995. Nat. Med.1, 267.
Ishiyama, M., et al. 1993. Chem. Pharm. Bull.41, 1118.
WST-1, 1-Methoxy PMS, and a user protocol.
法律資訊
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
免責聲明
Toxicity: Multiple Toxicity Values, refer to MSDS (O)
儲存類別代碼
10 - Combustible liquids
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商品
Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.
基于细胞的细胞增殖（BrdU、MTT、WST1）、细胞活力和细胞毒性实验，可应用于癌症、神经科学和干细胞研究。
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
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