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Merck

HCYTA-60K

MILLIPLEX® Human Cytokine/Chemokine/Growth Factor Panel A - Immunology Multiplex Assay

Synonym(s):

Human cytokine multiplex kit, Luminex® human cytokine immunoassay, Millipore human cytokine panel

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84
To order a Milliplex® kit, please search for your analyte of interest.
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species reactivity

human

Quality Level

200

manufacturer/tradename

Milliplex®

technique(s)

multiplexing: suitable

input

cell culture supernatant(s)
plasma
serum

detection method

fluorometric (Luminex® xMAP®)

shipped in

wet ice

storage temp.

2-8°C

Related Categories

General description

Cytokines, chemokines, and growth factors are key mediatorsof immune system functions capable of signaling through autocrine, paracrine,and endocrine mechanisms. Their pleiotropic immunomodulatory propertiesallow these biomolecules to react to diverse stimuli and regulate the immuneresponse, either promoting or inhibiting inflammation.

Application

  • MILLIPLEX® Qualified assays undergo rigorousassay development, verification, and Quality Control testing to achieve optimalperformance.
  • Simultaneously analyze up to 48 cytokine,chemokine, and growth factor biomarkers with bead-based multiplex assays usingLuminex technology, in human serum, plasma and cell culture samples.
  • Available analytes: sCD40L, EGF, Eotaxin, FGF-2,FLT-3L, Fractalkine, G-CSF, GM-CSF, GROα, IFNα2, IFNγ, IL-1α, IL-1β, IL-1RA,IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12 (p40), IL-12(p70), IL-13, IL-15, IL-17A, IL-17E/IL-25, IL-17F, IL-18, IL-22, IL-27, IP-10,MCP-1, MCP-3, M-CSF, MDC, MIG, MIP-1α, MIP-1β, PDGF-AA, PDGF-AB/BB, RANTES,TGFα, TNFα, TNFβ, VEGF-A

Features and Benefits

  • This panel is fully configurable, with options for custom bead premixing, fixed premixed and bulk premixed panels.
  • Save time and resources by simultaneously analyzing up to 48 analytes of your choice in a single sample
  • Requires a small sample volume (25 µL/well)
  • Choose same day or overnight incubation
  • Reliable and accurate performance with proven lot-to-lot consistency
  • Ready-to-use reagents
  • Comes with everything you need to run your assay
  • Includes a Serum Matrix to mimic the native sample environment in serum and plasma samples
  • Includes high and low Quality Controls

Packaging

96-well plate

Preparation Note

Recommended storage for kit components is 2 - 8°C.

Legal Information

Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalogor other company documentation accompanying the product(s), our products areintended for research use only and are not to be used for any other purpose,which includes but is not limited to, unauthorized commercial uses, in vitrodiagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumptionor application to humans or animals.

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Signal Word

Danger

Hazard Classifications

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2

Target Organs

Respiratory Tract

Storage Class Code

10 - Combustible liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Liam J O'Neil et al.
Frontiers in immunology, 13, 958145-958145 (2022-09-27)
The development of autoantibody directed towards citrullinated proteins (ACPA) are predictive of RA in at-risk individuals. The biological events that underpin loss of immune tolerance and progression into inflammatory arthritis are not known. We sought to identify serum proteomic alterations
Myung-Ho Kim et al.
The Journal of infectious diseases (2021-05-27)
We analyzed the plasma levels of interferons and cytokines, and the expression of interferon-stimulated genes in peripheral blood mononuclear cells in COVID-19 patients with different disease severity. Mild patients exhibited transient type I interferon responses, while ICU patients had prolonged
Qi Wang et al.
Frontiers in immunology, 13, 995223-995223 (2022-09-27)
In primary biliary cholangitis (PBC), the levels of serum IL-2 were involved in liver inflammation and immune changes. This study aimed to investigate the prognostic significance of serum IL-2 combined with total bilirubin (TBIL) in liver failure and cytokine changes
Teslin S Sandstrom et al.
Journal of virology, 95(9) (2021-02-12)
The use of unique cell surface markers to target and eradicate HIV-infected cells has been a longstanding objective of HIV-1 cure research. This approach, however, overlooks the possibility that intracellular changes present within HIV-infected cells may serve as valuable therapeutic
Jinyu Li et al.
Frontiers in immunology, 12, 749618-749618 (2021-12-11)
Increasing cases related to the pathogenicity of Enterovirus D68 (EV-D68) have made it a growing worldwide public health concern, especially due to increased severe respiratory illness and acute flaccid myelitis (AFM) in children. There are currently no vaccines or medicines
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Questions

1–9 of 9 Questions  

  1. Could you provide a recommendation for sample preparation for placenta and HCYTA-60K assay?

    1 answer

    1. The Milliplex assays have not been tested or verified for use with placenta tissue homogenates. However, a reference is available using placenta homogenates in the Human Cytokine Panel 1 (HCYTOMAG-60K).

      The samples were prepared as follows:
      Placental homogenate: Fragments of frozen placental tissue were transferred to propylene tubes containing lysis buffer and a cocktail of protease inhibitors. The placenta samples were homogenized using an electric homogenizer on ice. After homogenization, the homogenates were centrifuged, and the protein concentration in the supernatant was measured and stored for further analysis.

      Reference: Molás RB, Ribeiro MR, Ramalho Dos Santos MJC, et al. The involvement of annexin A1 in human placental response to maternal Zika virus infection Antiviral Res. 2020 Jul; 179:104809.

      Helpful?

  2. What type of IL-12p40 was detected in the Human Cytokine Panel A (HCYTA-60K) – IL-12p40 monomer or IL-12p40 total (both monomer and homodimer)?

    1 answer

    1. The capture in the assay detects the IL-12p40 monomer, and the standard used is also the IL-12p40 monomer. However, specific testing has not been conducted to determine if it also detects the homodimer.

      Helpful?

  3. Are the standards used in Human Cytokine Panel A expressed in E. Coli?

    1 answer

    1. The IFNa2 protein is expressed in yeast, while IL-12p40, IL-12p70, and IL-27 proteins are expressed in HEK293. All the other proteins are expressed in E. Coli.

      Helpful?

  4. When precipitating proteins (albumin and globulins) from plasma or serum samples using an Acetonitrile solution, does cortisol, which is bound to albumin & globulin, dissociate into the solution or get removed along with the protein precipitate?

    1 answer

    1. The treatment with acetonitrile removes the matrix effect, including the proteins, from the samples. This means that the cortisol bound to albumin and other proteins is eliminated. The assay specifically measures the free cortisol (non-bound cortisol).

      Helpful?

  5. Can the Milliplex Human Cytokine/Growth Factor Panel A (HCYTA-60K) measure IFNa2a or IFNA2B?

    1 answer

    1. Based on the antibody and standard specification sheets, the kit detects both IFNa2a and IFNa2B. The capture antibody is listed as an IFNa2B antibody and does not mention IFNa2a. However, the standard is recombinant IFNa2a.

      Helpful?

  6. Could you please provide the expected sample ranges for the Milliplex Human Cytokine Panel A (HCYTA-60K)?

    1 answer

    1. The serum and plasma sample values for the HCYTA-60K kit are provided. It's important to note that these values should be used as a guideline only. This data is based on a small sample size without effort for randomization and is not a true reference range.

      Helpful?

  7. Is it better to use EDTA vacutainers than heparinized vacutainers when collecting blood from plasma? Does this apply to both humans and mice?

    1 answer

    1. For our Milliplex assays, EDTA plasma is preferred. Care must be taken when using heparin as an anticoagulant since an excess of heparin will provide falsely high values. Use no more than 10 IU heparin per mL of blood collected.

      Helpful?

  8. Do you have any information about BPA, specifically bisphenol A from the plastic used in the immunoassay kits?

    1 answer

    1. The plastic materials used in the immunoassay kit are BPA-free according to the manufacturer's websites.

      Helpful?

  9. Is there an alternative method if a bath sonicator is not available for sonication of beads as mentioned in the Milliplex protocols? Can vortexing for a longer period serve as an alternative?

    1 answer

    1. The Milliplex assays recommend using a sonicator (water bath, Branson B200 or equivalent). Sonication helps break up any aggregated beads and beads stuck to the vial to reduce aggregation and improve counts. If a bath sonicator is not available, you can vortex for up to 2 minutes.

      Helpful?

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