A protocol is not available for this compound.
Please see the link below to review a publication that may be helpful.
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7712457/
35845
DCFHDA
BioReagent, suitable for fluorescence, ≥95% (HPLC)
Synonym(s):
2′,7′-Dichlorofluorescein diacetate, Diacetyldichlorofluorescein
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About This Item
Empirical Formula (Hill Notation):
C24H14Cl2O7
CAS Number:
Molecular Weight:
485.27
UNSPSC Code:
12352108
NACRES:
NA.32
PubChem Substance ID:
MDL number:
Beilstein/REAXYS Number:
68488
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Product Name
DCFHDA, BioReagent, suitable for fluorescence, ≥95% (HPLC)
InChI key
VQVUBYASAICPFU-UHFFFAOYSA-N
InChI
1S/C24H14Cl2O7/c1-11(27)30-21-9-19-15(7-17(21)25)24(14-6-4-3-5-13(14)23(29)33-24)16-8-18(26)22(31-12(2)28)10-20(16)32-19/h3-10H,1-2H3
SMILES string
CC(=O)Oc1cc2Oc3cc(OC(C)=O)c(Cl)cc3C4(OC(=O)c5ccccc45)c2cc1Cl
Quality Level
description
suitable as a fluorogenic substrate for esterases
product line
BioReagent
assay
≥95% (HPLC)
form
powder or crystals
mol wt
485.27 g/mol
color
faint red
white to faint brown
mp
232-234 °C (lit.)
solubility
DMSO: soluble
fluorescence
λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase)
suitability
suitable for fluorescence
storage temp.
2-8°C
Related Categories
1 of 4
This Item | 21884 | 35848 | D6883 |
|---|---|---|---|
| suitability suitable for fluorescence | suitability suitable for fluorescence | suitability suitable for fluorescence | suitability - |
| fluorescence λex 504 nm; λem 524 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 470 nm; λem 529 nm in 0.1 M Tris pH 8.0 (esterase) | fluorescence λex 504 nm; λem 529 nm in 0.1 M Tris pH 8.0 | fluorescence - |
| solubility DMSO: soluble | solubility DMF: soluble, DMSO: soluble, methanol: soluble | solubility - | solubility - |
| assay ≥95% (HPLC) | assay ≥85% (HPCE) | assay ≥90% (T) | assay ≥97% |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. - | storage temp. −20°C |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
Application
General description
2′,7′-Dichlorofluorescein diacetate, also known as DCFHDA or Dcfh-DA, is a sensitive cell-permeable redox probe. The lipophilic non-fluorescent Dcfh-DA readily crosses the cell membrane through passive diffusion followed by deacetylation. The deacylated product is an oxidant sensitive 2′,7′-dichlorofluorescein (DCHF). DCHF is oxidized later to form highly fluorescent DCF, which is measured.[1]
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Gloves
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2',7'-Dichlorodihydrofluorescein diacetate (DCFH-DA) is commonly used to detect the generation of reactive oxygen intermediates and for assessing the overall oxidative stress in toxicological phenomenon. It has been suggested that DCFH-DA crosses the cell membrane, subsequently undergoing deacetylation by intracellular esterases.
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Can I get the protocol? Thank you
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What is the shelf life of this product, as a powder, and once dissolved?
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This product is not assigned an expiration date or recommended retest date. Products with no expiration date or recommended retest date should be routinely inspected by customers to ensure they perform as expected. These products are also subject to a one year warranty from the date of shipment. For more information you may access the "Product Dating Information" document under "ADDITIONAL USEFUL DOCUMENTS ABOUT OUR PRODUCTS" at the bottom of the Quality Services page with this link: https://www.sigmaaldrich.com/life-science/quality-and-regulatory-management/quality-services.
Stock solutions of this item in organic solvents are stable for at least 6 months if stored at -20°C.
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Can Product 35845, 2´,7´-Dichlorofluorescein diacetate be used for ROS (Reactive Oxygen Species) detection?
1 answer-
Yes, Product 35845, 2´,7´-Dichlorofluorescein diacetate can be used for ROS detection by FACS analysis.
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In what solvents is 2´,7´-´Dichlorofluorescein diacetate, Product 35845, soluble?
1 answer-
It is soluble in DMF, ethanol (2 mg/mL), DMSO (10 mg/mL).
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What are the recommended storage conditions for 2´\,7´-Dichlorofluorescein diacetate, Product 35845?
1 answer-
Product no. 35845 should be stored in a refrigerator or coldroom at 2-8°C.
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What is required to get 2´,7´-Dichlorofluorescein diacetate, Product 35845, to fluoresce?
1 answer-
2',7'-dichlorofluorescein diacetate fluoresces only after cleavage of the acetyl groups.
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What are the optical properties of the hydrolysis product of 2´,7´-Dichlorofluorescein diacetate, Product 35845?
1 answer-
Excitation maximum is at 504 nm; emission maximum is at 529 nm (in 0.1 M Tris pH 8.0 following treatment with an esterase).
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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