D4138

Deoxyribonuclease II from porcine spleen

Name: Deoxyribonuclease II from porcine spleen
Brand: SIGMA

Type IV, lyophilized powder, 2,000-6,000 Kunitz units/mg protein (biuret)

Synonym(s):

DNase II, Deoxyribonucleate 3′-oligonucleotido-hydrolase

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About This Item

CAS Number:

9025-64-3

EC Number:

3.1.22.1 (BRENDA, IUBMB)

EC Number:

232-801-8

MDL number:

MFCD00130919

UNSPSC Code:

12352204

NACRES:

NA.54

Key Documents

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type

Type IV

Quality Level

200

form

lyophilized powder

specific activity

2,000-6,000 Kunitz units/mg protein (biuret)

mol wt

45 kDa

composition

Protein, 60-80%

foreign activity

RNase ≤0.05%

storage temp.

−20°C

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This Item	D8764	D4513	D4527
Sigma-Aldrich D4138 Deoxyribonuclease II from porcine spleen	Sigma-Aldrich D8764 Deoxyribonuclease II from bovine spleen	Sigma-Aldrich D4513 Deoxyribonuclease I from bovine pancreas	Sigma-Aldrich D4527 Deoxyribonuclease I from bovine pancreas
specific activity 2,000-6,000 Kunitz units/mg protein (biuret)	specific activity ≥1,000 units/mg protein	specific activity ≥2,000 units/mg protein	specific activity ≥2,000 units/mg protein
form lyophilized powder	form essentially salt-free, lyophilized powder	form lyophilized powder	form lyophilized powder
mol wt 45 kDa	mol wt 38 kDa	mol wt ~31 kDa	mol wt ~31 kDa
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 300	Quality Level 300
foreign activity RNase ≤0.05%	foreign activity RNase ≤0.1%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%	foreign activity Chymotrypsin ≤0.01%, Protease ≤0.005%, RNase ≤0.01%

Application

DNase II from Sigma has been used to treat transformed cells during the purification of β-lactamase.[1] It has also been used for the preparation of adenoma tissue in a study that investigated the effect of somatoprim on growth hormone secretion in human adenoma cell cultures (hSA).[2]

Deoxyribonuclease II from porcine spleen has been used in an immunohistological study of the immune system cells in paraffin-embedded tissues. Deoxyribonuclease II from porcine spleen has also been used in a study to investigate its reassociation with the lysosomal membrane.

Biochem/physiol Actions

The enzyme is a lysosomal acid hydrolase that hydrolyzes DNA to 3′-phosphoryl oligonucleotides in the absence of metal ions, under acidic conditions.[3] It is a non-covalently linked α·β heterodimer of approximately 45,000 Da molecular mass.[4]

Physical form

Contains sodium chloride

Other Notes

One Kunitz unit will produce a ΔA₂₆₀ of 0.001 per min per mL at pH 4.6 at 25°C; [Mg²⁺] = 0.83 mM

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Porcine spleen deoxyribonuclease II. Covalent structure, cDNA sequence, molecular cloning, and gene expression.

C C Wang et al.

The Journal of biological chemistry, 273(27), 17192-17198 (1998-06-27)

Porcine spleen DNase II, a lysosomal acid hydrolase, is a noncovalently linked alpha.beta heterodimer (Liao, T.-H. (1985) J. Biol. Chem. 260, 10708-10713). The alpha subunit, after disulfide cleavage, yields two chains, alpha1 and alpha2. The complete amino acid sequences of

Immunohistological study of the immune system cells in paraffin-embedded tissues of conventional pigs.

F Chianini et al.

Veterinary immunology and immunopathology, 82(3-4), 245-255 (2001-10-06)

The distribution of different cells of the immune system has been studied in formalin-fixed paraffin-embedded tissues from conventionally reared healthy pigs, using immunohistological techniques. The samples collected were: lungs, tonsils, lymph nodes (mediastinal, mesenteric, inguinal and submandibular), pancreas, spleen, liver

pKa measurements from nuclear magnetic resonance of tyrosine-150 in class C beta-lactamase.

Yoko Kato-Toma et al.

The Biochemical journal, 371(Pt 1), 175-181 (2003-01-07)

13C-NMR spectroscopy was used to estimate the p K a values for the Tyr(150) (Y150) residue in wild-type and mutant class C beta-lactamases. The tyrosine residues of the wild-type and mutant lactamases were replaced with (13)C-labelled L-tyrosine ([ phenol -4-(13)C]tyrosine)

DG3173 (somatoprim), a unique somatostatin receptor subtypes 2-, 4- and 5-selective analogue, effectively reduces GH secretion in human GH-secreting pituitary adenomas even in Octreotide non-responsive tumours.

U Plöckinger et al.

European journal of endocrinology, 166(2), 223-234 (2011-11-09)

Somatostatin analogues (SSA) reduce autonomous GH secretion by activating somatostatin receptors (sst) 2 and 5 in 50-60% of acromegalic patients. However, by inhibiting insulin secretion these SSA reduce glucose tolerance. DG3173 is a novel SSA with additional binding to sst4

Horizontal gene transfer: you are what you eat.

Lars Holmgren

Biochemical and biophysical research communications, 396(1), 147-151 (2010-05-25)

Horizontal or lateral gene transfer is an effective mechanism for the exchange of genetic information in bacteria allowing bacterial diversification and facilitating adaptation to new environments. Recent data demonstrate that DNA may also be transferred between somatic cells via the

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