05-797R

Anti-phospho-Erk1/2 (Thr202/Tyr204, Thr185/Tyr187)Antibody, recombinant clone AW39R, rabbit monoclonal

clone AW39R, Upstate^®, from rabbit

• Synonym(s): Erk1, Erk2, p44-MAPK, p42-MAPK, MAPK2, PRKM2, p41mapk, PRKM1, p44, ERK2, P42MAPK, ERK, p42, MAPK1, ERT1, ERT2, Mitogen-activated protein kinase, MAP Kinase 2, MAP Kianse 1, MAPK
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: AW39R, monoclonal
• species reactivity: human, mouse, rat
• manufacturer/tradename: Upstate^®
• technique(s): multiplexing: suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NP_002736; NP_002737
• UniProt accession no.: P27361; P28482
• shipped in: dry ice
• target post-translational modification: phosphorylation ((pThr202/pTyr204), (pThr185/pTyr187))
• Gene Information: human ... MAPK3(5595) ; mouse ... Mapk3(26417) ; rat ... Mapk3(50689)

Description

General description

Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).

Erk1 (p44) (~44 kDa) and Erk2 (p42) (~42 kDa).

Immunogen

Epitope: pTEpY in the activation loop

Phosphorylated peptide corresponding to amino acids surrounding the pTEpY motif in the activation loop of human phospho-Erk1/2, in which the Thr and Tyr residues are phosphorylated

Application

Anti-phospho-Erk1/2 (Thr202/Tyr204 Antibody, Thr185/Tyr187), recombinant clone AW39R detects level of phospho-Erk1/2 (Thr202/Tyr204 & has been published & validated for use in Mplex & WB.

Research Category
Signaling

Research Sub Category
MAP Kinases

Kinases & Phosphatases

Biochem/physiol Actions

Recognizes Erk 1 & 2 only when dually phosphorylated on its TxY activation motif.

Physical form

Format: Purified

Protein A Purified immunoglobulin in 30% glycerol, 0.07M Tris-glycine, pH 7.4, 0.105 M NaCl, 0.035% sodium azide as a preservative.

Protein A purified

Preparation Note

Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Control
PDGF-treated NIH/3T3 cell lysates

Routinely evaluated by immunoblot on lysate from PDGF-treated NIH/3T3 cells

Other Notes

Replaces: 05-797

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable