48-660MAG

MILLIPLEX MAPK/SAPK Signaling 10-Plex Kit - Cell Signaling Multiplex Assay

• Synonym(s): Cell Signaling Kit, MAPK/SAPK 10-Plex Kit
• UNSPSC Code: 12161503
• eCl@ss: 32161000
• NACRES: NA.47

Properties

• species reactivity: human
• Quality Level: 200
• manufacturer/tradename: Milliplex^®
• technique(s): multiplexing: suitable
• assay range: inter-assay cv: 4-13%; intra-assay cv: 3-10%
• detection method: fluorometric (Luminex^® xMAP^® technology)
• storage temp.: 2-8°C

Description

General description

Cells respond to their environment in various ways through intracellular signaling. Signaling through Receptor Tyrosine kinases (RTKs) often promotes increased metabolism and cell growth. Activation of ERK/MAP kinase is one of the key serine/threonine kinases activated via RTK signaling, promoting increased activity of p70 S6 kinase, MSK1, STATs, CREB, and many other signaling intermediates. Other signaling pathways induced via stress (ex. heat shock/arsenite treatment) or death receptors (ex. TNFα) promote p38, JNK, and p53 signaling pathways.

Application

• An overnight (4°C) incubation is recommended for best results.
• This assay requires 25 μL diluted cell lysate.
• This kit must be run using Assay Buffer 2 (provided).
• 1 - 25 μg cell lysate/well (recommended starting concentration is 20 μg cell lysate/well).

Intracellular Bead-Based Multiplex Assays using the Luminex technology enables the simultaneous relative quantitation of multiple phosphorylation or total pathway proteins in tissue and cell lysate samples.

Research Category
Signaling

Used to detect/quantify:ATF2 (Thr71)|Erk (Thr185/Tyr187)|HSP27 (Ser78)|JNK (Thr183/Tyr185)|c-Jun (Ser73)|MEK1 (Ser222)|MSK1 (Ser212)|p38 (Thr180/Tyr182)|p53 (Ser15)|STAT1 (Tyr701)

Biochem/physiol Actions

Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.

Legal Information

Luminex is a registered trademark of Luminex Corp

MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany

xMAP is a registered trademark of Luminex Corp

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial

Safety Information

• Pictograms: GHS05,GHS07,GHS09
• Signal Word: Danger
• Hazard Statements: H302,H315,H318,H410
• Precautionary Statements: P264 - P273 - P280 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Skin Irrit. 2
• Storage Class Code: 10 - Combustible liquids