Skip to Content
Merck

D8001

3,3′-Diaminobenzidine

97% (HPLC)

Synonym(s):

3,3′,4,4′-Biphenyltetramine, 3,3′,4,4′-Tetraaminobiphenyl, DAB

Slide 1 of 5
Sign In to View Organizational & Contract Pricing.

Select a Size

1 G

€27.20

5 G

€91.80

10 G

€172.00

25 G

€370.00

€27.20

Please contact Customer Service for Availability
Request a Bulk Order

About This Item

Linear Formula:
(NH2)2C6H3C6H3(NH2)2
CAS Number:
91-95-2
Molecular Weight:
214.27
UNSPSC Code:
12171500
NACRES:
NA.47
PubChem Substance ID:
24894175
EC Number:
202-110-6
Beilstein/REAXYS Number:
1212988
MDL number:
MFCD00007725

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Product Name

3,3′-Diaminobenzidine, 97% (HPLC)

InChI key

HSTOKWSFWGCZMH-UHFFFAOYSA-N

InChI

1S/C12H14N4/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8/h1-6H,13-16H2

SMILES string

NC1=C(N)C=CC(C2=CC(N)=C(N)C=C2)=C1

assay

97% (HPLC)

form

powder

mp

175-177 °C (lit.)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

Quality Level

200

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
D12384D4418D4293
3,3′-Diaminobenzidine 97% (HPLC)

Sigma-Aldrich

D8001

3,3′-Diaminobenzidine

3,3′-Diaminobenzidine 97%

Sigma-Aldrich

D12384

3,3′-Diaminobenzidine

SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL

Sigma-Aldrich

D4418

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL

Sigma-Aldrich

D4293

SIGMAFAST™ 3,3′-Diaminobenzidine tablets

assay

97% (HPLC)

assay

97%

assay

-

assay

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

mp

175-177 °C (lit.)

mp

175-177 °C (lit.)

mp

-

mp

-

storage temp.

room temp

storage temp.

-

storage temp.

−20°C

storage temp.

−20°C

form

powder

form

powder

form

tablet

form

tablet

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

-

application(s)

-

Application

3,3′-Diaminobenzidine has been used as a chromogen for the visualization in immunohistochemistry of chondrosarcoma sections,[1] mammary carcinomas[2] and brain tissue sections.[3]
Peroxidase substrate; reagent for spectrophotometric determination of selenium.

Biochem/physiol Actions

DAB (3,3′-Diaminobenzidine) is utilized in many applications for the visualization of peroxidase activity. In the peroxidase reaction, DAB serves as a hydrogen donor in the presence of peroxide. The oxidized DAB forms an insoluble brown end-product for use in the immunohistological and immunoblotting staining procedures.[4][5][6][7]

Other Notes

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 2

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Gloves

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
BCCN6906
CDBG6898
BCCM7438
BCCM1482
BCCM5088

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Motor skill training promotes sensorimotor recovery and increases microtubule-associated protein-2 (MAP-2) immunoreactivity in the motor cortex after intracerebral hemorrhage in the rat
Santos MV, et al.
ISRN neurology, 2013 (2013)
C M Escobedo-Bonilla et al.
Diseases of aquatic organisms, 74(2), 85-94 (2007-04-17)
White spot syndrome virus (WSSV) causes disease and mortality in cultured and wild shrimp. A standardized WSSV oral inoculation procedure was used in specific pathogen-free (SPF) Litopenaeus vannamei (also called Penaeus vannamei) to determine the primary sites of replication (portal
Metastatic canine mammary carcinomas can be identified by a gene expression profile that partly overlaps with human breast cancer profiles
Klopfleisch R, et al.
BMC Cancer, 10(1), 618-618 (2010)
A colorimetric method for measurement of the (peroxidase-mediated) oxidation of 3,3'-diaminobenzidine.
Fahimi HD and Herzog V
The Journal of Histochemistry and Cytochemistry, 21, 499-502 (1973)
Detection of T lymphocytes in intestine of broiler chicks treated with Lactobacillus spp. and challenged with Salmonella enterica serovar Enteritidis.
Noujaim JC, et al.
Poultry Science, 87, 927-933 (2008)
View All Related Papers

Articles

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service