C0733

Cyclooxygenase 1 from sheep

Name: Cyclooxygenase 1 from sheep
Brand: SIGMA
Price: 1010 EUR

glycerol solution, ≥1500 units/mg protein

Synonym(s):

COX-1, Constitutive cyclooxygenase, Prostaglandin H synthase 1, Prostaglandin endoperoxide synthase

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5000 UNITS

€1,010.00

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About This Item

UNSPSC Code:

12352204

NACRES:

NA.32

EC Number:

1.14.99.1 (BRENDA, IUBMB)

MDL number:

MFCD02098124

Key Documents

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Product Name

Cyclooxygenase 1 from sheep, glycerol solution, ≥1500 units/mg protein

form

glycerol solution

specific activity

≥1500 units/mg protein

mol wt

dimer subunit mol wt 70 kDa

UniProt accession no.

O78749

shipped in

dry ice

storage temp.

−70°C

Quality Level

200

Gene Information

sheep ... COX1(808251)

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This Item	C0858	SRP0523	SRP0524
Sigma-Aldrich C0733 Cyclooxygenase 1 from sheep	Sigma-Aldrich C0858 Cyclooxygenase 2 human	Sigma-Aldrich SRP0523 COX1, FLAG^® -His-Tags	Sigma-Aldrich SRP0524 COX2, FLAG^® -His-Tags
Gene Information sheep ... COX1(808251)	Gene Information human ... PTGS2(5743)	Gene Information human ... COX1(6775083)	Gene Information human ... COX2(6775079)
specific activity ≥1500 units/mg protein	specific activity ≥8000 units/mg protein	specific activity -	specific activity -
form glycerol solution	form aqueous solution	form frozen liquid	form frozen liquid
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −70°C	storage temp. −70°C	storage temp. −70°C	storage temp. −70°C
mol wt dimer subunit mol wt 70 kDa	mol wt dimer subunit mol wt 70-74 kDa	mol wt 70.5 kDa	mol wt 71 kDa

Application

Cyclooxygenase 1 from sheep has been used as positive control protein in western blot analysis of osteoarthritis samples[1] and in prostaglandin synthase activity assay.[2]

Biochem/physiol Actions

COX-1 catalyzes the conversion of arachidonic acid to prostaglandin H₂ (the first step in the biosynthesis of prostaglandins, thromboxanes, and prostacyclins). It is involved in the homeostatic role of eicosanoids and constitutively expressed in almost all animal tissues. Has an apparent K_M of 8.3 μM for arachidonic acid.

General description

Cyclooxygenase 1 is a 71 kDa membrane bound protein predominantly present in endoplasmic reticulum. It has three domains, the epidermal growth factor (EGF) like domain, enzymatic and membrane binding domain.[3] Cyclooxygenase 1 mediates prostaglandin synthesis and is modulated by anti inflammatory nonsteroidal drugs.[4]

Other Notes

One unit consumes one nanomole of oxygen per minute at 37 °C in 0.1 M Tris-HCl buffer, pH 8, containing 100 μM arachidonate, 5 mM EDTA, 2 mM phenol, and 1 μM hematin.

Physical form

Solution in 80 mM Tris-HCl, pH 8, with 0.1% TWEEN^® 20, 300 μM diethyldithiocarbamate and 10% glycerol.

Legal Information

TWEEN is a registered trademark of Croda International PLC

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number

0000502148

0000488922

0000476985

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The Sigma class glutathione transferase from the liver fluke Fasciola hepatica

LaCourse EJ, et al.

PLoS Neglected Tropical Diseases, 6(5), e1666-e1666 (2012)

Prostaglandin hydroperoxidase, an integral part of prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes.

S Ohki et al.

The Journal of biological chemistry, 254(3), 829-836 (1979-02-10)

The highly purified prostaglandin endoperoxide synthetase from bovine vesicular gland microsomes had two still unresolved enzyme activities; the oxygenative cyclization of 8,11,14-eicosatrienoic acid to produce prostaglandin G1 and the conversion of the 15-hydro-peroxide of prostaglandin G1 to a 15-hydroxyl group

Cyclo-oxygenase isoenzymes: physiological and pharmacological role

Kam PCA and See AUL

Anaesthesia, 55(5), 442-449 (2000)

Novel analgesic/anti-inflammatory agents: 1,5-diarylpyrrole nitrooxyalkyl ethers and related compounds as cyclooxygenase-2 inhibiting nitric oxide donors.

Maurizio Anzini et al.

Journal of medicinal chemistry, 56(8), 3191-3206 (2013-03-29)

A series of 3-substituted 1,5-diarylpyrroles bearing a nitrooxyalkyl side chain linked to different spacers were designed. New classes of pyrrole-derived nitrooxyalkyl inverse esters, carbonates, and ethers (7-10) as COX-2 selective inhibitors and NO donors were synthesized and are herein reported.

Expression and activity of COX-1 and 2 and 5-LOX in joint tissues from dogs with naturally occurring coxofemoral joint osteoarthritis

Lascelles BD, et al.

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society, 27(9), 1204-1208 (2009)

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Cyclooxygenase 1 from sheep