SRP2083

WT-1 (-KTS) human

recombinant, expressed in insect cells, ≥60% (SDS-PAGE)

• Synonym(s): AWT1, GUD, NPHS4, WAGR, WIT-2, WT33
• UNSPSC Code: 12352200
• NACRES: NA.77

Properties

• biological source: human
• recombinant: expressed in insect cells
• Assay: ≥60% (SDS-PAGE)
• form: frozen liquid
• mol wt: ~55.6 kDa
• packaging: pkg of 5 μg
• storage condition: avoid repeated freeze/thaw cycles
• concentration: 250 μg/mL
• color: clear colorless
• NCBI accession no.: NM_000378
• UniProt accession no.: P19544
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... WT1(7490)

Description

General description

Wilms tumor 1 (WT1) is encoded by the gene mapped to human chromosome 11p13.[1] The encoded protein is expressed at high levels in nephrons, but at low levels in gonads, visceral adipose tissue, mesothelium and bone marrow cells.[2][3] In addition, it is also abundantly expressed in renal podocytes in adults and in parietal epithelial cells during early days.[3]

Biochem/physiol Actions

WT-1, the product of Wilms′ tumor suppressor gene Wt1, is a nuclear protein with structural motifs characteristic of transcription factors, including four C-terminal zinc fingers. While different pre-mRNA processing could result in 16 isoforms of the protein, inclusion or exclusion of exon 5 and the three amino acids (KTS) between zinc fingers 3 and 4 largely affects the activity of WT1 protein. Such a complex post-transcriptional regulation, particularly in splicing, may represent a major regulatory mechanism for tumorigenesis of the Wilms′ tumor. WT1 (-KTS) appears to have different binding affinity to both DNA and RNA compared to the +KTS form.

Wilms tumor 1 (WT1) is involved in various vertebrate developmental processes such as cell differentiation and proliferation, apoptosis and regulation of epithelial/mesenchymal characteristics.[2] It serves as a tumor suppressor gene but might also possess oncogenic property.[4] The encoded protein regulates mRNA splicing and interactions between protein. It is associated with cancers and as well as kidney function.[2] Mutations in the gene leads to Denys-Drash syndrome, resulting in renal and genitourinary tract abnormalities.[3] Deletion in the germline WT1 gene causes Wilms′ tumor. This gene is highly expressed in a various types of cancers.[4]

Physical form

Clear and colorless frozen liquid solution

Preparation Note

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. While working, please keep sample on ice.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable