577801

Anti-Tau Mouse mAb (TAU-5)

liquid, clone TAU-5, Calbiochem^®

• Synonym(s): Anti-Tau antibody
• NACRES: NA.43
• UNSPSC Code: 12352203

Properties

• Product Name: Anti-Tau Mouse mAb (TAU-5), liquid, clone TAU-5, Calbiochem^®
• biological source: mouse
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: TAU-5, monoclonal
• form: liquid
• does not contain: preservative
• species reactivity: mouse, human, sheep, rat
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• dilution: (Frozen Sections (1-2 µg/mL); Immunoblotting (1 µg/mL); Immunoprecipitation (10 µg per 200-500 µg cell lysate); Paraffin Sections (1-2 µg/mL, heat pre-treatment required))
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −70°C
• target post-translational modification: unmodified
• Quality Level: 100
• Gene Information: human ... MAPT(4137) ; mouse ... Mapt(17762) ; rat ... Mapt(29477)

Description

Application

Frozen Sections (1-2 µg/ml)

Immunoblotting (1 µg/ml)

Immunoprecipitation (10 µg per 200-500 µg cell lysate)

Paraffin Sections (1-2 µg/ml, heat pre-treatment required)

Disclaimer

Toxicity: Standard Handling (A)

General description

Protein G purified mouse monoclonal antibody. Recognizes the ~45-68 kDa phosphorylated and unphosphorylated forms of tau.

Recognizes the ~45-68 kDa Tau protein.

This Anti-Tau Mouse mAb (TAU-5) is validated for use in Frozen Sections, Immunoblotting, Immunoprecipitation, Paraffin Sections for the detection of Tau.

Immunogen

Bovine

Epitope: within the central region

purified bovine microtubule-associated proteins

Other Notes

Papasozomenos, S.C. and Shanavas, A., 2002. Proc. Natl. Acad. Sci. USA99, 1140.
Rapoport, M., eta al. 2002. Proc. Natl. Acad. Sci. USA99, 6364.

When used for formalin/paraffin embedded sections, staining is enhanced by boiling tissue sections in 10 mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at room temperature for 20 min prior to antibody incubation. Alternate splicing of tau mRNA and differential phosphorylation contribute to the heterogeneity of tau. Does not cross-react with other MAPS of tubulin. Recognizes both native and phosphorylated forms of tau. Variables associated with assay conditions will dictate proper working dilution.

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In PBS.

Preparation Note

Following initial thaw, aliquot and freeze (-70°C). Do not store diluted antibody without carrier protein if the concentration is <50 µg/ml.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable