MABF120

Anti-EGFR Antibody, clone 225 (Azide-free)

Name: Anti-EGFR Antibody, clone 225 (Azide-free)
Brand: MM
Price: 1722 ILS

clone 225, from mouse

Synonym(s):

Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

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100 μG

₪1,722.00

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About This Item

UNSPSC Code:

12352203

NACRES:

NA.41

eCl@ss:

32160702

Key Documents

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Product Name

Anti-EGFR Antibody, clone 225 (Azide-free), clone 225, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

225, monoclonal

species reactivity

human

technique(s)

immunoprecipitation (IP): suitable
neutralization: suitable

isotype

IgG1κ

NCBI accession no.

NP_005219

UniProt accession no.

P00533

shipped in

dry ice

target post-translational modification

unmodified

Quality Level

100

Gene Information

human ... EGFR(1956)

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Show Differences

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This Item	MABS835	MABS829	SAB4500714
Sigma-Aldrich MABF120 Anti-EGFR Antibody, clone 225 (Azide-free)	Sigma-Aldrich MABS835 Anti-EGFR Antibody, clone 7F12.1	Sigma-Aldrich MABS829 Anti-phospho-EGFR (Tyr1173) Antibody, clone 3E7.2	Sigma-Aldrich SAB4500714 Anti-EGFR antibody produced in rabbit
biological source mouse	biological source mouse	biological source mouse	biological source rabbit
Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956)	Gene Information human ... EGFR(1956)
clone 225, monoclonal	clone 7F12.1, monoclonal	clone 3E7.2, monoclonal	clone polyclonal
antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form purified immunoglobulin	antibody form affinity isolated antibody
species reactivity human	species reactivity human	species reactivity human	species reactivity human, rat, mouse
isotype IgG1κ	isotype IgG2bκ	isotype IgG2bκ	isotype -

Analysis Note

Evaluated by Immunoprecipitation in A431 cell lysate.

Immunoprecipitation Analysis: 10 µg of this antibody immunoprecipitated EGFR from A431 cell lysate.

Application

Anti-EGFR Antibody, clone 225 (Azide-free) is an antibody against EGFR for use in Immunoprecipitation, Neutralizing.

Neutralizing Assay Analysis: A representative lot was used by an independent laboratory in A431 cells. (Kawamoto, T., et al. (1984). Journal of Biological Chemistry. 259(12):7761-7766.)

General description

The epidermal growth factor receptor (EGFR; Proto-oncogene c-ErbB-1) is a ubiquitously receptor tyrosine kinase that is activated by a variety of ligands including EGF, EPGN, TGFA, and EREG. Ligand-bound EGFR undergoes dimerization and autophosphorylation exposing cytoplasmic domains which allow interaction with adaptor proteins and induction of several signaling pathways including PI3-AKT; PLCγ-PKC; Notch1; and Ras-Raf. EGFR therefore contributes to a diverse range of cellular processes inlcuding cell proliferation. Several studies have reported that EGFR signaling plays an important role in the development of various tumors. EGFR is regulated by PTPRJ and PTPRK phosphatases, and by the endocytic machinery including EPS15 that mediates internalization and degradation of the receptor.

~170 kDa observed. Uniprot gives a calculated molecular weight of 135 but can be observed at ~170 kDa due to phosphorylation. (Konishi, A., et al. (2003). The Journal of Biological Chemistry. 278(37):35049–35056.)

Immunogen

Partially purified human EGFR from A431 cells

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-338

Physical form

Format: Purified

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Storage Class

12 - Non Combustible Liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Journal of translational medicine, 17(1), 201-201 (2019-06-20)

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