XTG9-RO
Roche
X-tremeGENE™ 9 DNA Transfection Reagent
Polymer reagent for transfecting common cell lines
Recommended Products
grade
for molecular biology
Quality Level
form
liquid (aqueous solution)
usage
mL (suitable for 165 transfections)
packaging
pkg of 0.4 mL (06365779001)
pkg of 1.0 mL (06365787001)
pkg of 5 × 1.0 mL (06365809001)
manufacturer/tradename
Roche
technique(s)
transfection: suitable
storage temp.
2-8°C
Related Categories
General description
Application
X-tremeGENE™ 9 DNA Transfection Reagent is well suited for cellular analysis applications such as:
- Expression of recombinant proteins for functional analysis.
- Physiological studies of metabolic pathways.
- Analysis of regulatory sequences using reporter gene assays.
- Gene expression assays.
- Cancer research studies.
- Target evaluation.
Features and Benefits
- Generate physiologically relevant results using a reagent with extremely low cytotoxicity for maximum post-transfection cell viability.
- Save time and eliminate multiple handling steps; simply dilute X-tremeGENE 9 DNA Transfection Reagent, incubate with plasmid DNA, and pipet the mixture directly onto your cells (with or without serum).
- Avoid time-consuming optimization procedures in commonly used cell lines.
Quality
Physical form
Other Notes
Legal Information
related product
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
334.4 °F
Flash Point(C)
168 °C
Certificates of Analysis (COA)
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Articles
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.
Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.
This brief webinar provides an overview of what transfection is and the methods that are used to introduce DNA or RNA into eukaryotic cells.
Protocols
Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).
Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.
Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents
Related Content
Browse our convenient transfection reagent selection guide to match the best reagent for your specific cell line and application needs.
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