XTGHP-RO

X-tremeGENE^™ HP DNA Transfection Reagent

High-performance polymer reagent for transfecting many cell lines

• Synonym(s): transfection reagent
• NACRES: NA.55
• UNSPSC Code: 41106502
• eCl@ss: 32040201

Properties

• grade: Molecular Biology
• form: liquid (aqueous solution)
• usage: mL (suitable for 165 transfections)
• packaging: pkg of 0.4 mL (06366244001), pkg of 1.0 mL (06366236001), pkg of 5 × 1 mL (06366546001)
• manufacturer/tradename: Roche
• technique(s): transfection: suitable
• storage temp.: −20°C

Description

General description

X-tremeGENE HP DNA Transfection Reagent is a multi-component reagent that forms a complex with DNA , then transports the complex into animal or insect cells.

Application

X-tremeGENE^™ HP has been used for efficient transfection during lentivirus production to generate stable MARCH6 knockout cell lines

Features and Benefits

X-tremeGENE HP DNA Transfection Reagent is a high performance reagent that transfects a broad range of eukaryotic cells, including insect cells and hard-to-transfect cell lines (e.g., Sf9, U-2 OS, MEF, MCF7, Hep G2, PC-3, HeLa, HT-29, HT-1080, K-562, RAW 264.7, Jurkat, PC-12, and HCT 116).

• Benefit from an easy-to-use non-liposomal reagent that is free of animal-derived components, stable at room temperature, filtered through 0.2 μm pore size membrane, and active in serum-containing medium.
• Achieve new levels of transfection efficiency in primary cells and tumor cell lines that are not transfected well by other reagents.
• Generate physiologically relevant data using a reagent with low cytotoxic effects.
• Increase experimental throughput and enable target evaluation using a simple and consistent protocol.

Physical form

Supplied in 80% ethanol and sterile-filtered through 0.2 μm pore size membrane. Does not contain any ingredients of human or animal origin.Number of Tests: Using the standard procedure, 1 ml of X-tremeGENE HP DNA Transfection Reagent can be used to perform up to 10,000 transfections in 96-well plates.

Analysis Note

Each lot of X-tremeGENE HP DNA Transfection Reagent is carefully tested following established quality procedures to ensure that the product is consistently performing according to specifications. During quality testing, cells are transfected with a reporter gene vector DNA using X-tremeGENE HP DNA Transfection Reagent (ratio 3:1 ¼l/¼g DNA). Reporter gene activity is monitored via chemiluminescent detection. Using a standard curve, the total amount of recombinant protein is determined per well in order to meet specification.

Other Notes

For life science research only. Not for use in diagnostic procedures.

Legal Information

X-tremeGENE is a trademark of Roche

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Precautionary Statements: P210 - P233 - P280 - P303 + P361 + P353 - P337 + P313 - P370 + P378
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2
• Storage Class: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point (°F): 62.2 °F
• Flash Point (°C): 16.8 °C
• Hazard Codes: H225,H319