67309

Kovac′s reagent for indoles

suitable for microbiology

• Synonym(s): 4-(Dimethylamino)benzaldehyde solution
• Empirical Formula (Hill Notation): C₉H₁₁NO
• CAS Number: 100-10-7
• Molecular Weight: 149.19
• UNSPSC Code: 41171621
• NACRES: NA.85
• PubChem Substance ID: 329760541
• MDL number: MFCD00003381
• Beilstein/REAXYS Number: 4132845

Properties

• Product Name: Kovac′s reagent for indoles, suitable for microbiology
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
• SMILES string: [H]C(=O)c1ccc(cc1)N(C)C
• InChI key: BGNGWHSBYQYVRX-UHFFFAOYSA-N
• agency: according to ISO 16654:2001
• product line: BioChemika
• shelf life: limited shelf life, expiry date on the label
• composition: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• technique(s): microbe id | specific enzyme detection: suitable
• application(s): agriculture; clinical testing; environmental; food and beverages; microbiology
• storage temp.: 2-8°C
• suitability: Escherichia coli; coliforms
• Quality Level: 200

Description

Application

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent.[1] It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.[2]

General description

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid.[3] For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.[4]

Safety Information

• pictograms: GHS02,GHS05,GHS07
• signalword: Danger
• hcodes: H226,H290,H314,H317,H332,H335
• pcodes: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 3 - Flammable liquids
• wgk: WGK 3
• flash_point_f: 109.4 °F
• flash_point_c: 43 °C
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter