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For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf
A4718
Agarose
Molecular Biology
Synonym(s):
3,6-Anhydro-α-L-galacto-β-D-galactan
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₪1,231.00
₪2,827.00
About This Item
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Product Name
Agarose, Molecular Biology
SMILES string
O1[C@H]([C@@H]([C@H]([C@H]([C@H]1CO)O)O[C@@H]4O[C@@H]5[C@H]([C@@H](OC5)[C@@H]4O)O[C@@H]6O[C@@H]([C@@H]([C@@H]([C@H]6O)O)O)CO)O)O[C@H]2[C@H]3OC[C@@H]2O[C@H]([C@H]3O)O
InChI
1S/C24H38O19/c25-1-5-9(27)11(29)12(30)22(38-5)41-17-8-4-36-20(17)15(33)24(40-8)43-18-10(28)6(2-26)39-23(14(18)32)42-16-7-3-35-19(16)13(31)21(34)37-7/h5-34H,1-4H2/t5-,6-,7+,8+,9+,10+,11+,12-,13+,14-,15+,16-,17-,18+,19+,20+,21-,22+,23+,24+/m1/s1
InChI key
MJQHZNBUODTQTK-WKGBVCLCSA-N
biological source
algae (marine)
grade
Molecular Biology
form
powder
technique(s)
electrophoresis: suitable
EEO
≤0.05
mp
≤75 °C (3% gel)
transition temp
gel point ≤35 °C (3% gel)
foreign activity
DNase, RNase, NICKase, none detected
storage temp.
room temp
Quality Level
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Related Categories
1 of 4
This Item | A2576 | A9539 | A6013 |
|---|---|---|---|
| EEO ≤0.05 | EEO ≤0.12 | EEO 0.09-0.13 | EEO 0.09-0.13 |
| form powder | form powder | form powder | form powder |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| grade for molecular biology | grade Molecular Biology | grade for molecular biology | grade - |
| technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable | technique(s) electrophoresis: suitable |
| foreign activity DNase, RNase, NICKase, none detected | foreign activity - | foreign activity DNase, RNase, none detected | foreign activity - |
Analysis Note
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.
Application
Low ethidium bromide and SYBR Green background staining
General description
Storage Class
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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How can I determine the shelf life / expiration / retest date of this product?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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Is this agarose high-resolution, and suitable for PCR applications?
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The High Resolution Agarose, product number A4718, is an intermediate-melting agarose that offers approximately double the resolution capability compared to standard agarose. It is capable of resolving PCR products and DNA fragments ranging from 200-800 bp that differ in size by as little as 2%. This makes it ideal for separating small DNA fragments, such as PCR products, with very small size differences, offering a resolution comparable to that achieved with polyacrylamide gels.
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