94149

Atto 647N-Streptavidin

BioReagent, suitable for fluorescence

• Synonym(s): Streptavidin-Atto 647N
• UNSPSC Code: 12161900
• NACRES: NA.25

Properties

• product line: BioReagent
• Quality Level: 100
• form: powder
• manufacturer/tradename: ATTO-TEC GmbH
• λ: in ethanol (with 0.1% trifluoroacetic acid)
• UV absorption: λ: 650.0-656.0 nm A_max
• suitability: corresponds for gel electrophoresis; suitable for fluorescence
• storage temp.: −20°C

Description

General description

Atto 647N is a new generation fluorescent dye dedicated towards the red spectral region. It is a cationic dye and carries a net electrical charge of +1 post substrate coupling reaction. Streptavidin, isolated from Streptomyces avidinii, is a tetrameric protein of 4 x 13.2 kDa which binds very tightly to the small molecule biotin. The dissociation constant of the complex is extremely small (Kd ≈ 10-15 M), ranking among the strongest non-covalent interactions. Atto streptavidin conjugates are readily soluble in water.

Application

Atto 647N-Streptavidin is designed for application in the area of life science, e.g., labeling of DNA, RNA or proteins. Atto streptavidin conjugates may be used as secondary detection reagents in flow cytometry, immunoassays, blot analysis, and histochemical applications. Atto 647N -Streptavidin is suitable for multiplex analysis, including multicolor imaging.

Features and Benefits

Atto 647N- Streptavidin has the following benefits-

• Highly photostable. Strong absorption.
• Excellent fluorescent quantum yield.
• Highly resistant to ozone degradation.
• Minimal triplet formation.

Analysis Note

dye-to-protein ratio ≥1.5

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves