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A7005

β-Amylase from sweet potato

greener alternative

Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

Synonym(s):

1,4-α-D-Glucan maltohydrolase

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About This Item

CAS Number:
9000-91-3
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-566-1
MDL number:
MFCD00081391
EC Number:
3.2.1.2 (BRENDA, IUBMB)

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Product Name

β-Amylase from sweet potato, Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

biological source

sweet potato

type

Type I-B

form

ammonium sulfate suspension

specific activity

≥750 units/mg protein (E1%/280)

greener alternative product score

old score: 57
new score: 1
Find out more about DOZN™ Scoring

greener alternative product characteristics

Waste Prevention
Safer Solvents and Auxiliaries
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

≥7.0 mg/mL

greener alternative category

, Re-engineered

storage temp.

2-8°C

Quality Level

200

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1 of 4

This Item
A8781A8220A9857
β-Amylase from sweet potato Type I-B, ammonium sulfate suspension, ≥750 units/mg protein (E1%/280)

Sigma-Aldrich

A7005

β-Amylase from sweet potato

β-Amylase from sweet potato

Sigma-Aldrich

A8781

β-Amylase from sweet potato

α-Amylase from Aspergillus oryzae aqueous solution, ≥800 FAU/g

Sigma-Aldrich

A8220

α-Amylase from Aspergillus oryzae

α-Amylase from Aspergillus oryzae ≥150 units/mg protein (biuret)

Sigma-Aldrich

A9857

α-Amylase from Aspergillus oryzae

biological source

sweet potato

biological source

-

biological source

Aspergillus sp. (A. oryzae)

biological source

-

specific activity

≥750 units/mg protein (E1%/280)

specific activity

-

specific activity

≥800 FAU/g

specific activity

≥150 units/mg protein (biuret)

form

ammonium sulfate suspension

form

powder

form

aqueous solution

form

powder

concentration

≥7.0 mg/mL

concentration

-

concentration

-

concentration

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

Quality Level

200

Quality Level

100

Quality Level

200

Quality Level

200

Application

β -Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. β -Amylase, from Sigma, has been used in various plant studies, such as carbon starvation studies in Populus tremuloides.[1] β-amylase from sweet potato has been used to examine the utility of the enzyme in inhibiting and removing Staphylococcus aureus biofilms.[2] The enzyme has also been used to prepare β-limit dextrin from waxy maize starch.[3]
β-Amylase from sweet potato has been used:
  • in the hydrolysis of starch and starch-pectin combinations[4]
  • for comparing amylases from different sources in Staphylococcus aureus SH1000 biofilm inhibition and reduction assay[2]
  • for selenium speciation in samples[5]

Biochem/physiol Actions

β-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. β-amylase from sweet potato is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry.[6]
β-Amylases plays a major role in examining enzymatic promiscuity among glycosidases.[7]

General description

β-Amylases belongs to the class of amylolytic enzymes.[7]
We are committed to bringing you Greener Alternative Products, which adhere to one of the four categories of Greener Alternatives . This product belongs to category of Re-engineered products, showing key improvements in Green Chemistry Principles “Waste Prevention”, “Safer Solvents and Auxiliaries” and “Enhanced Energy Efficiency”. Click here to view its DOZN scorecard.
We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Other Notes

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 4.8 at 20 °C.
View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Physical form

Crystalline suspension in 2.3 M (NH4)2SO4

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

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Certificates of Analysis (COA)

Lot/Batch Number
0000429463
0000429463
0000409467
0000409467
0000378834

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Mechanisms of enzymatic glycosyl transfer
Comprehensive Natural Products II (2010)
Effect of cross-linking on the resistance to enzymatic hydrolysis of waxy maize starch and low-methoxy pectin
Khondkar D, et al.
Food Hydrocolloids, 23(2), 387-393 (2009)
Xin Qi et al.
Journal of pharmacy & pharmaceutical sciences : a publication of the Canadian Society for Pharmaceutical Sciences, Societe canadienne des sciences pharmaceutiques, 14(1), 60-66 (2011-04-20)
β-Limit dextrin has been studied for many years as a means to investigate the internal structures of amylose and amylopectin. However its role as an excipient in the pharmaceutical industry has never been reported. This paper is the first one
C G Cheong et al.
Proteins, 21(2), 105-117 (1995-02-01)
Sweet potato beta-amylase is a tetramer of identical subunits, which are arranged to exhibit 222 molecular symmetry. Its subunit consists of 498 amino acid residues (Mr 55,880). It has been crystallized at room temperature using polyethylene glycol 1500 as precipitant.
Bradford Craigen et al.
The open microbiology journal, 5, 21-31 (2011-07-16)
Staphylococcus aureus, a versatile human pathogen, is commonly associated with medical device infections. Its capacity to establish and maintain these infections is thought to be related to its ability to form adherent biofilms. In this study, commercially available α-amylase compounds
View All Related Papers

Articles

How to Work with Enzymes Supplied as Ammonium Sulfate Suspensions

Instructions for working with enzymes supplied as ammonium sulfate suspensions

Protocols

Enzymatic Assay of β-AMYLASE (EC 3.2.1.2)

Beta-amylase is found in bacteria, fungi, and plants. To measure β-amylase activity, this assay uses a colorimetric spectrophotometric stop reaction at 540 nm.

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