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C3142
α-Chymotrypsin from bovine pancreas
(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein
Synonym(s):
TLCK-Chymotrypsin
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| Size/SKU | Availability | Price |
|---|---|---|
10 mg | Please contact Customer Service for Availability | ₪428.00 |
25 mg | Please contact Customer Service for Availability | ₪857.00 |
100 mg | Please contact Customer Service for Availability | ₪1,959.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
eCl@ss:
42010112
EC Number:
232-671-2
NACRES:
NA.54
MDL number:
EC Number:
Specific activity:
≥40 units/mg protein
Assay:
≥90% (UV)
Biological source:
bovine pancreas
₪428.00
Please contact Customer Service for Availability
Quality Segment
biological source
bovine pancreas
type
Type VII
assay
≥90% (UV)
form
essentially salt-free, lyophilized powder
specific activity
≥40 units/mg protein
mol wt
25 kDa
optimum pH
7.8
solubility
1 mM HCl: soluble 10 mg/mL, clear
UniProt accession no.
storage temp.
−20°C
Gene Information
cow ... CTRB1(618826)
General description
Substrate Specificity
Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa
Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa
Application
Biochem/physiol Actions
α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca2+ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α1-antitrypsin, α2-macroglobulin, 10 mM Cu2+ and Hg2+.
A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.
Preparation Note
TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.
Analysis Note
Protein determined by A1%/280
Other Notes
One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.
View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer
1 of 1
This Item | |||
|---|---|---|---|
| description (TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein | description Type I-S, essentially salt-free, lyophilized powder | description Type II, lyophilized powder, ≥40 units/mg protein | description suitable for protein sequencing, salt-free, lyophilized powder |
| Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) | Gene Information cow ... CTRB1(618826) |
| specific activity ≥40 units/mg protein | specific activity ≥40 units/mg protein | specific activity ≥40 units/mg protein | specific activity - |
| assay ≥90% (UV) | assay - | assay ≥85% (UV) | assay - |
| form essentially salt-free, lyophilized powder | form essentially salt-free, lyophilized powder | form lyophilized powder | form salt-free, lyophilized powder |
| mol wt 25 kDa | mol wt 25 kDa | mol wt 25 kDa | mol wt - |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. - |
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Signal Word
Danger
Hazard Codes
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
Target Organs
Respiratory system
Storage Class
11 - Combustible Solids
WGK
WGK 1
PPE (personal protective equipment)
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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What is the Department of Transportation shipping information for this product?
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How can solutions of alpha-chymotrypsin (Product No. C3142) be stored?
1 answer-
Stock solutions prepared in 1 mM HCl, containing 2 mM calcium chloride, can be stored at -20 ° for about one week.
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What is the pH optimum for the enzyme, Product No. C3142?
1 answer-
The pH optimum for alpha-chymotrypsin is between pH 7.5-8.5. Please see the following reference: Wilcox, P.E., Chymotrypsingogens-chymotrypsins, Methods in Enzymology, 19, 64-80 (1970).
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What is the molecular weight of alpha-chymotrypsin from bovine pancreas (Product No. C3142)?
1 answer-
The molecular weight of alpha chymotrypsin, reported in the literature, is approximately 25 kDa.This information and the basic structure description of the enzyme can be found on our product information sheet (under Documents, above) at our website.
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How do you recommend preparing a solution of Product No. C3142?
1 answer-
It is recommended to reconstitute Product No. C3142 in 1 mM hydrochloric acid containing 2 mM calcium chloride. The calcium functions as both a stabilizer (and possibly an activator) of the enzyme.
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