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Merck

F5883

4-Fluoro-7-nitrobenzofurazan

≥98% (elemental analysis)

Synonym(s):

NBD-F

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₪1,768.00

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₪5,706.00

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₪9,292.00

₪1,768.00

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About This Item

Empirical Formula (Hill Notation):
C6H2FN3O3
CAS Number:
29270-56-2
Molecular Weight:
183.10
UNSPSC Code:
12352100
NACRES:
NA.32
PubChem Substance ID:
24894897
MDL number:
MFCD00010196
Beilstein/REAXYS Number:
1077571

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Product Name

4-Fluoro-7-nitrobenzofurazan, ≥98% (elemental analysis)

InChI

1S/C6H2FN3O3/c7-3-1-2-4(10(11)12)6-5(3)8-13-9-6/h1-2H

SMILES string

[O-][N+](=O)c1ccc(F)c2nonc12

InChI key

PGZIDERTDJHJFY-UHFFFAOYSA-N

assay

≥98% (elemental analysis)

mp

52-54 °C (lit.)

fluorescence

λex 380 nm; λem 515 nm (hydrolyzed (esterase))

storage temp.

2-8°C

Quality Level

100

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Show Differences

1 of 4

This Item
4714025455F3639
4-Fluoro-7-nitrobenzofurazan ≥98% (elemental analysis)

Sigma-Aldrich

F5883

4-Fluoro-7-nitrobenzofurazan

4-Fluoro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥98.0% (HPLC)

Sigma-Aldrich

47140

4-Fluoro-7-nitrobenzofurazan

4-Chloro-7-nitrobenzofurazan BioReagent, suitable for fluorescence, ≥97.0% (HPLC)

Sigma-Aldrich

25455

4-Chloro-7-nitrobenzofurazan

4-Fluoro-7-sulfamoylbenzofurazan

Sigma-Aldrich

F3639

4-Fluoro-7-sulfamoylbenzofurazan

fluorescence

λex 380 nm; λem 515 nm (hydrolyzed (esterase))

fluorescence

λex 470 nm; λem 528 nm in ethanol, λex 470 nm; λem 550 nm (Amino acid adducts)

fluorescence

λex 336 nm in methanol, λex 420 nm; λem ~540 nm in ethanol (after derivatization with glycine)

fluorescence

-

Quality Level

100

Quality Level

200

Quality Level

100

Quality Level

200

assay

≥98% (elemental analysis)

assay

≥98.0% (HPLC)

assay

≥97.0% (HPLC)

assay

-

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

storage temp.

−20°C

mp

52-54 °C (lit.)

mp

52-54 °C (lit.)

mp

97-100 °C, 97-99 °C (lit.)

mp

-

Application

Useful for fluorescent labeling of amino acids and amines in HPLC

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

pictograms

Exclamation mark
GHS07

signalword

Warning

hcodes

H315,H319

Hazard Classifications

Eye Irrit. 2 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

235.4 °F - closed cup

flash_point_c

113 °C - closed cup

ppe

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCF9196
1405141V
1405141
048012/1
117H2600

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Yang-Ki Park et al.
Journal of chromatography. A, 1217(20), 3357-3361 (2010-04-13)
Single drop microextraction (SDME) can be in-line coupled with capillary electrophoresis by attaching a drop to the tip of a capillary. With a 2-layer drop comprised of an aqueous basic acceptor phase covered with a thin organic layer, acidic analytes
Yanting Song et al.
Amino acids, 42(5), 1897-1902 (2011-04-21)
A high-performance liquid chromatography method in which fluorescence detection is used for the simultaneous determination of 21 amino acids is proposed. Amino acids were derivatized with 4-fluoro-7-nitro-2,1,3-benzoxadiazole (NBD-F) and then separated on a monolithic silica column (MonoClad C18-HS, 150 mm×3
Nasr Y Khalil
Talanta, 80(3), 1251-1256 (2009-12-17)
A fully automated, rapid and highly sensitive HPLC method with automated sample pre-treatment by column-switching system and fluorescence detection has been developed for the trace quantitative determination of the new antidepressant reboxetine (RBX) in human plasma. A simple pre-column derivatization
Chiaki Aoyama et al.
Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 25(1), 63-65 (2009-01-14)
A simple and sensitive determination method for selenomethionine (Se-Met) using an HPLC-fluorescence detection system coupled with an on-line electrochemical reactor has been developed. NBD-F (4-fluoro-7-nitro-2,1,3-benzoxadiazole) was used as the fluorescent derivatization reagent for Se-Met. NBD-Se-Met was separated from NBD-derivatives of
Hua-Ming Tseng et al.
Electrophoresis, 30(8), 1399-1405 (2009-03-26)
A sensitive CE with LIF method has been developed for quantitative analysis of small carbohydrates. In this work, 17 carbohydrates including mono-, di- and oligosaccharides were simultaneously derivatized with 4-fluoro-7-nitrobenzofurazane (NBD-F) via a two-step reaction involving reductive amination with ammonia
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