No, if using a 95% ethanol step before eosin staining, it is important to avoid dragging water into the alcoholic eosin solution. Introducing too much water might make the solution more alkaline, which can affect the staining. pH is not typically measured as it tends to stain the pH electrode. Additionally, because this is a colored solution, using pH papers is not a viable option. If staining is poor, the solution either needs to be replaced, or it is possible to add 0.5 mL of glacial acetic acid per 100 ml of eosin Y solution to improve the staining.
HT110116
Eosin Y Solution, Alcoholic
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500 ML
₪385.00
About This Item
NACRES:
NA.47
UNSPSC Code:
41116124
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form
solution
shelf life
Expiry date on the label.
IVD
for in vitro diagnostic use
concentration
0.5 % (w/v) in acidified ethanol
application(s)
hematology
histology
storage temp.
room temp
Quality Level
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This Item | HT110132 | HT110180 | HT1101128 |
|---|---|---|---|
| concentration 0.5 % (w/v) in acidified ethanol | concentration 0.5 % (w/v) in acidified ethanol | concentration 0.5 % (w/v) in acidified ethanol | concentration 0.5 % (w/v) in acidified ethanol |
| application(s) hematology | application(s) hematology | application(s) hematology | application(s) hematology |
| form solution | form solution | form solution | form solution |
| storage temp. room temp | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| Quality Level 500 | Quality Level 500 | Quality Level 500 | Quality Level 500 |
| shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. | shelf life Expiry date on the label. |
Application
General purpose cytoplasmic counterstain. Used with hematoxylin and eosin staining.
Other Notes
Certified Eosin Y, 0.5% (w/v) in acidified 90% ethanol
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - Skin Sens. 1
Storage Class
3 - Flammable liquids
wgk
WGK 2
flash_point_f
55.4 °F - closed cup
flash_point_c
13 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
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Does the Eosin Y alcoholic solution (HT1101) need to be pH tested regularly?
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