O3756

Orange G

for NA electrophoresis

• Synonym(s): 1-Phenylazo-2-naphthol-6,8-disulfonic acid disodium salt, 7-Hydroxy-8-phenylazo-1,3-naphthalenedisulfonic acid disodium salt, Acid Orange 10, Wool Orange 2G
• Empirical Formula (Hill Notation): C₁₆H₁₀N₂Na₂O₇S₂
• CAS Number: 1936-15-8
• Molecular Weight: 452.37
• Colour Index Number: 16230
• Beilstein: 4120705
• EC Number: 217-705-6
• MDL number: MFCD00012457
• UNSPSC Code: 12171500
• PubChem Substance ID: 57654483
• NACRES: NA.52

Properties

• grade: Molecular Biology
• form: powder
• technique(s): nucleic acid detection: suitable
• solubility: water: 1 mg/mL, clear, orange to red
• suitability: suitable for gel electrophoresis
• storage temp.: room temp
• SMILES string: [Na+].[Na+].Oc1ccc2cc(cc(c2c1\N=N\c3ccccc3)S([O-])(=O)=O)S([O-])(=O)=O
• InChI: 1S/C16H12N2O7S2.2Na/c19-13-7-6-10-8-12(26(20,21)22)9-14(27(23,24)25)15(10)16(13)18-17-11-4-2-1-3-5-11;;/h1-9,19H,(H,20,21,22)(H,23,24,25);;/q;2*+1/p-2/b18-17+;;
• InChI key: HSXUHWZMNJHFRV-QIKYXUGXSA-L

Description

General description

Orange G is an azo dye[1] that is acidic in nature.[2] It is widely used as a tracking dye for nucleic acid gel electrophoresis to track DNA front in agarose gels. Typically runs at 50bp in up to 2.5% agarose.

Application

Orange G has been used as a DNA gel loading dye.[3][4][5]

Orange G is a tracking dye used in nucleic acid electrophoresis to track DNA front in agarose gels. Also used in SDS-capillary electrophoresis as a standard. Typically runs at 50bp in up to 2.5% agarose.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves