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Merck

P1526

o-Phenylenediamine dihydrochloride

peroxidase substrate, chromogenic, solid

Synonym(s):

OPD

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₪730.00

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₪1,332.00

50 G

₪2,269.00

100 G

₪3,781.00

1 KG

₪18,221.00

₪730.00

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About This Item

Linear Formula:
C6H4(NH2)2·2HCl
CAS Number:
615-28-1
Molecular Weight:
181.06
NACRES:
NA.83
PubChem Substance ID:
24898213
UNSPSC Code:
12352204
EC Number:
210-418-7
MDL number:
MFCD00012966
Beilstein/REAXYS Number:
3912045

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Product Name

o-Phenylenediamine dihydrochloride, peroxidase substrate

InChI

1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H

InChI key

RIIWUGSYXOBDMC-UHFFFAOYSA-N

SMILES string

Cl[H].Cl[H].Nc1ccccc1N

form

solid

color

white to off-white

mp

258 °C (dec.) (lit.)

storage temp.

−20°C

Quality Level

300

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o-Phenylenediamine dihydrochloride peroxidase substrate

Sigma-Aldrich

P1526

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 1 mg substrate per tablet

Sigma-Aldrich

P6662

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 5 mg substrate per tablet

Sigma-Aldrich

P3804

o-Phenylenediamine dihydrochloride

o-Phenylenediamine dihydrochloride tablet, 15 mg substrate per tablet

Sigma-Aldrich

P4664

o-Phenylenediamine dihydrochloride

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

200

form

solid

form

tablet

form

tablet

form

tablet

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

color

white to off-white

color

-

color

-

color

-

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

mp

258 °C (dec.) (lit.)

Application

o-Phenylenediamine dihydrochloride has been used as a peroxidase substrate in enzyme-linked immunosorbent assay (ELISA) procedures.[1][2][3][4]

Disclaimer

May darken in storage.

General description

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

signalword

Danger

Hazard Classifications

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Sens. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
0000518779
0000518778
0000518781
0000518782
0000518781

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Areano Ethério Moreira de Farias et al.
Tropical animal health and production, 51(4), 745-752 (2018-11-14)
Goat breeding in the Northeast region of Brazil plays an important socioeconomic role. However, there are significant losses caused by sanitary deficits and infectious diseases, particularly caseous lymphadenitis (CL). Although CL is considered endemic in Northeastern Brazil, a comprehensive and
Andrew K I Falconar et al.
Clinical and vaccine immunology : CVI, 13(9), 1044-1051 (2006-09-09)
We compared dengue virus (DV) isolation rates and tested whether acute primary (P) and acute/probable acute secondary (S/PS) DV infections could be correctly classified serologically when the patients' first serum (S1) samples were obtained 1 to 3 days after the
Bikash R Sahu et al.
The Journal of infectious diseases, 198(3), 434-443 (2008-07-01)
Filaria-specific antibodies of immunoglobulin (Ig) G, IgE, and IgM isotypes have been correlated with acquired immunity in the literature, but the status of filaria-specific IgA and its role in human filariasis has not been addressed. The present study attempts to
E O'Connor et al.
Journal of immunological methods, 229(1-2), 155-160 (1999-11-11)
The ability to detect a protein is always limited to the sensitivity of the assays available. Progress in improving the sensitivity of protein detection will allow a more complete understanding of biological systems. Of particular interest to the field of
Guy Mollett et al.
Clinical infectious diseases : an official publication of the Infectious Diseases Society of America, 69(7), 1130-1135 (2018-12-13)
Visceral leishmaniasis (VL), caused by the Leishmania donovani complex, is a fatal, neglected tropical disease that is targeted for elimination in India, Nepal, and Bangladesh. Improved diagnostic tests are required for early case detection and for monitoring the outcomes of
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