UHPLC/MS Analysis of Steroids on Titan C18

column

Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)

mobile phase

(A) water; (B) acetonitrile, both with 0.1% formic acid

gradient

40%B for 1 min, to 90%B in 0.5 min, held for 2 min

flow rate

0.4 mL/min

pressure

4500 psi (310 bar)

detector

MS, ESI(+), MRM

injection

2.0 μL