91228

Trichloroacetic acid

BioUltra, ≥99.5% (T)

• Synonym(s): TCA
• Linear Formula: Cl₃CCOOH
• CAS Number: 76-03-9
• Molecular Weight: 163.39
• UNSPSC Code: 12352101
• NACRES: NA.25
• PubChem Substance ID: 57653428
• EC Number: 200-927-2
• Beilstein/REAXYS Number: 970119
• MDL number: MFCD00004177

Properties

• Product Name: Trichloroacetic acid, BioUltra, ≥99.5% (T)
• InChI key: YNJBWRMUSHSURL-UHFFFAOYSA-N
• InChI: 1S/C2HCl3O2/c3-2(4,5)1(6)7/h(H,6,7)
• SMILES string: OC(=O)C(Cl)(Cl)Cl
• grade: biotech. grade
• vapor density: <1 (vs air)
• vapor pressure: 1 mmHg ( 51 °C)
• product line: BioUltra
• assay: ≥99.5% (T)
• form: crystals; flakes
• impurities: insoluble matter, passes filter test
• ign. residue: ≤0.05%
• refractive index: n20/D 1.62 (lit.)
• pH: <1.0 (25 °C, 0.5 M in H₂O)
• bp: 196 °C (lit.)
• mp: 54-58 °C (lit.)
• solubility: H₂O: 0.5 M at 20 °C, clear, colorless
• density: 1.62 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤30 mg/kg; nitrate (NO₃^-): ≤20 mg/kg; phosphate (PO₄^3-): ≤10 mg/kg; sulfate (SO₄^2-): ≤100 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• λ: 0.5 M in H₂O
• UV absorption: λ: 260 nm A_max: 1.60; λ: 280 nm A_max: 0.15
• Quality Level: 200

Description

Application

Traditionally used to precipitate protein. Has been used to determine protein concentration by quantitative precipitation. Used as decalcifier and fixative in microscopy.

Trichloroacetic acid (TCA) has been used:

• to determine its effect on avian embryos during an exposure window encompassing cardiac specification
• as a cell fixative
• to terminate the O-acetylserine (thiol)lyase (OASTL) enzymatic assay
• for the precipitation of acidic ribosomal proteins (RPs)

General description

Trichloroacetic acid (TCA) is derived from Trichloroethylene (TCE) metabolism. It is used as an acid decalcifying agent. TCA is used as a fixative for nuclear staining and protein precipitation.

Other Notes

Concentration of protein by quantitative precipitation; Cytochemical demonstration of DNA with a thiazine dye.

Safety Information

• pictograms: GHS05,GHS09
• signalword: Danger
• hcodes: H314,H410
• pcodes: P260 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Corr. 1A
• Storage Class: 8B - Non-combustible corrosive hazardous materials
• wgk: WGK 2
• flash_point_f: >235.4 °F - closed cup
• flash_point_c: > 113 °C - closed cup
• ppe: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges