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Benzonase® endonuclease


Endonuclease from Serratia marcescens
CAS Number:
Enzyme Commission number:
MDL number:


suitable for biopharmaceutical production EMPROVE® bio

Quality Level

product line





8.0 (25 °C in H2O)

shipped in

dry ice

storage temp.

−20°C (−15°C to −25°C)

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General description

Benzonase® endonuclease acts as an unspecific endonuclease, degrading both DNA and RNA to small 3–5 base pairs (< 6 kDa) fragments with no base preference. It is the ideal tool for nucleic acid removal in virus vector and vaccine manufacturing as demanded by regulators. Additionally, the use of Benzonase® endonuclease increases the yield in virus purification, protects downstream chromatography and filter devices from fouling, and reduces feed stream viscosity.

Benzonase® endonuclease is the smart solution for DNA removal in viral vaccine and viral vector production and has proven its value for over 30 years. Balancing efficiency and regulatory compliance by delivering reliability and high-quality due manufacturing under good manufacturing practices (GMP ICH Q7). Our extensive documentation packages of Emprove® dossiers and the availability of a FDA Drug Master File type II, supports you in filing your vaccine manufacturing process to the authorities.


1 ea
500000 u in Alu tube
5000000 units


Used for the removal of nucleic acid from protein samples.

Biochem/physiol Actions

Digests native or heat-denatured DNA and RNA.

Analysis Note

Appearance (description): clear
Appearance (color): colourless
Activity (DNA; pH 8.0; 30 min; 37 °C): ≥ 250 U/µl
Spec. activity (calc. on protein): ≥ 1.1E+06 U/mg
Identity (electrophoresis): passes test
Purity (calc. on protein): ≥ 99.0%
Proteases: not detectable
Colony count (aerobic bacteria): <10 CFU in 100 000 U
Colony count (Yeasts and moulds): <10 CFU in 100 000 U
Endotoxines (LAL-test): <0.25 EU in 1000 U

Legal Information

Benzonase is a registered trademark of Merck KGaA, Darmstadt, Germany
Emprove is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

10 - Combustible liquids



Certificate of Analysis

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