1.05642
HPTLC plates, Silica gel 60 F₂₅₄
pkg of 50 plates, plate L × W 20 cm × 10 cm, glass support
Synonym(s):
HPTLC Silica Gel Plates
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About This Item
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material
glass support
silica gel 60 matrix
Quality Level
feature
binder Organic Polymer
fluorescent indicator
packaging
pkg of 50 plates
technique(s)
thin layer chromatography (TLC): suitable
layer thickness
200 μm
plate L × W
20 cm × 10 cm
particle size
5-6 μm
pore size
60 Å medium pore diameter
storage temp.
2-30°C
Related Categories
1 of 4
This Item | 1.05629 | 1.05628 | 1.05556 |
|---|---|---|---|
| material glass support, silica gel 60 matrix | material glass support, silica gel 60 matrix | material glass support, silica gel 60 matrix | material aluminum support, silica gel 60 matrix |
| technique(s) thin layer chromatography (TLC): suitable | technique(s) thin layer chromatography (TLC): suitable | technique(s) thin layer chromatography (TLC): suitable | technique(s) thin layer chromatography (TLC): suitable |
| pore size 60 Å medium pore diameter | pore size 60 Å medium pore diameter | pore size 60 Å medium pore diameter | pore size 60 Å medium pore diameter |
| feature binder Organic Polymer, fluorescent indicator | feature binder Organic Polymer, fluorescent indicator | feature binder Organic Polymer, fluorescent indicator | feature binder Organic Polymer, fluorescent indicator |
| packaging pkg of 50 plates | packaging pkg of 100 plates | packaging pkg of 25 plates | packaging pkg of 20 sheets |
| layer thickness 200 μm | layer thickness 200 μm | layer thickness 200 μm | layer thickness 200 μm |
General description
Application
Analysis Note
Pore volume (N₂-isotherm): 0.74 - 0.84 ml/g
d 50 (laser diffraction, size distribution): 5 - 7 µm
Layer thickness: 150 - 200 µm
Deviation of layer thickness per plate: ≤ 35 µm
Chromatographic testings:
A) Colour test
hRf-values
- bleu vif organol, colour test, lipophile:
17 - 22
- Ceres Black G, colour test, lipophile: 38 - 43
- ceres Violet brn, colour test, lipophile: 58 - 64
separation number
- colourtest, lipophile:
≥ 10.0
B) Steroid test
hRf-values
- hydrocortisone, steroid test:
23 - 29
- Reichstein S, steroid test: 36 - 45
- methyltestosterone, steroid test: 43 - 52
separation number
- steroid test:
≥ 10.5
Typical value determined on a conditioned plate
Eluent A) Toluene (45% rel.humidity)
Eluent B) Ethyl acetate/Toluene (95+5 v/v; 20% rel.humidity)
Other Notes
related product
Certificates of Analysis (COA)
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Articles
In this article we present several HPTLC applications and analytical standards for ginsenosides.
HPTLC plates provide superior separation, speed, and sensitivity, making them ideal for advanced qualitative and quantitative analysis.
Learn about our recently launched HPTLC calibration mix, developed in collaboration with CAMAG, that enables for an efficient and reliable universal system suitability testing (SST).
Development of an HPTLC based method, using Silica Gel 60 F254 plate, to determine Betamethasone Sodium Phosphate over a concentration range of 1 to 7 µg/spot.
Protocols
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HPTLC was used to analyze caffeine in coffee. Filtration of coffee through a PTFE syringe filter was the only sample preparation. Quantitation was conducted with a TLC scanner.
In the present study we demonstrate compliance of silica gel 60 F254 HPTLC plates with USP requirements, and how HPTLC plates are suitable for the Sildenafil citrate-Limit of Imidazole test.
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