Lugol's solution (product 1.09261) is specially formulated for use in Gram staining methods. There is no information on file regarding the use of this solution to stain cellulose fibers and analyzing by micro computed tomography. There is a review article from North Carolina State University that mentions various methods for staining cellulose fibers.
1.09261
Lugol′s solution (diluted iodine-potassium iodide solution)
for the Gram staining method
Synonym(s):
Iodine-potassium iodide solution
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Quality Level
form
liquid
IVD
for in vitro diagnostic use
technique(s)
microbe id | staining: suitable
pH
3.5 (20 °C in H2O)
bp
100 °C/1013 hPa
density
1.01 g/cm3 at 20 °C
application(s)
clinical testing
diagnostic assay manufacturing
hematology
histology
storage temp.
15-25°C
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This Item | 1.00567 | 32922 | 62650 |
|---|---|---|---|
| technique(s) microbe id | staining: suitable | technique(s) microbe id | staining: suitable | technique(s) - | technique(s) microbe id | staining: suitable |
| form liquid | form liquid | form liquid | form - |
| application(s) clinical testing | application(s) clinical testing | application(s) diagnostic assay manufacturing | application(s) - |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. room temp | storage temp. - |
| IVD for in vitro diagnostic use | IVD for in vitro diagnostic use | IVD - | IVD - |
| Quality Level 400 | Quality Level 400 | Quality Level 200 | Quality Level 200 |
General description
Lugol′s solution (diluted iodine-potassium iodide solution - for the Gram staining method, is a ready-to-use solution used in Bacteriology, for example, for the staining of Gram bacteria. In bacteriology, the Gram staining allows a fast differentiation of bacteria in Gram-positive and Gram-negative. The mureine structure of the bacteria wall is the basis of the color affinity. In the first step, bacteria will be stained with crystal violet (Product number 1.09218), an aniline dye. After the treatment with iodine solution (Lugol′s solution), a dye-iodine complex will form. During the decolorizing step (Gram′s decolorizing solution, Product number 1.10218), this complex stays in the multilayer mureine structure of the cell wall of Gram-positive bacteria - they will appear blue-violet. Gram-negative bacteria, by contrast, have a cell wall consisting of a single-layered murein structure, and correspondingly re-release the staining dye with the decoloring solution. Gram-negative bacteria will be counterstained with safranine solution (Product number 1.09217) and will then appear pink to red.
Lugol′s solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Lugol′s solution (diluted iodine-potassium iodide solution) is capable of staining up to 500 slides. This product is registered as IVD and CE marked. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
ISHL Indicated Name
Substances Subject to be Indicated Names
ISHL Notified Names
Substances Subject to be Notified Names
JAN Code
1092610000:
1092618502:
EC11092611000:
1092612500:
1092619023:
1092611000:
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Hi, Can we use this Lugol solution for staining of cotton fibres to get absorption contrast in cotton/viscose blended (both cellulose) yarn during micro computed tomography?
1 answer-
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Hi, is this a 1:1 mixture of KI and iodine solution in water? What's the concentration of each component, please?
1 answer-
Lugol's Solution is considered a weak or dilute iodine-potassium iodide soltuion. This product is intended for use in Gram staining. Although the exact concentration is considered proprietary, the Safety Data Sheet lists the Iodine concentration as greater than 0.1% but less than 1%. There is an expectation that the combined percentage of iodine and potassium iodide is approximately 1%. More concentrated solutions are not available at this time.
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