Unfortunately, the exact formulation is proprietary, however, it is simply a 5% Casein solution in 5X TBS. It does include a preservative at low concentrations.
70955
Casein
5%, Alkali-soluble, Unique blocking reagent for ultra-low background
Synonym(s):
blocking buffer, immunodetection blocking buffer
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This item can only be ordered on the Merck Millipore website.
About This Item
UNSPSC Code:
41105329
NACRES:
NB.23
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Product Name
5% Alkali-soluble Casein, Unique blocking reagent for ultra-low background
form
liquid
manufacturer/tradename
Novagen®
storage condition
OK to freeze
shipped in
ambient
storage temp.
2-8°C
1 of 4
This Item | C7594 | B6429 | G7663 |
|---|---|---|---|
| form liquid | form powder blend | form solution | form powder blend |
| shipped in ambient | shipped in - | shipped in - | shipped in - |
| storage temp. 2-8°C | storage temp. room temp | storage temp. room temp | storage temp. room temp |
| storage condition OK to freeze | storage condition - | storage condition - | storage condition - |
| manufacturer/tradename Novagen® | manufacturer/tradename - | manufacturer/tradename - | manufacturer/tradename - |
General description
Alkali-Soluble Casein is a superior blocking reagent for Western blot applications. This preparation minimizes non-specific background relative to other blocking reagents, such as BSA, gelatin, or non-fat milk. Alkali-Soluble Casein is effective in colorimetric and chemiluminescent detection protocols. Note that due to its high coating properties, detection sensitivity for some antibodies may be lower than with other blocking reagents under the same conditions (although the background is also reduced); the concentration can be optimized for maximal signal:noise ratios. Supplied as a 5% w/v solution; recommended working concentration range is 0.251%.
Unique blocking reagent for ultra-low background
Legal Information
NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Toxicity: Standard Handling (A)
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
70955-ML:
70955-3:
70955-150ML:
Certificates of Analysis (COA)
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Protocols
後続のウェスタンブロッティングのためのサンプル調製（培養組織・細胞の効果的な溶解とタンパク質抽出）プロトコル
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
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