AB5970

Anti-Dishevelled-1 Antibody

Chemicon^®, from rabbit

• Synonym(s): DSH, DVL1, DSH Homolog 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): western blot: suitable
• input: sample type hematopoietic stem cell(s); sample type neural stem cell(s)
• NCBI accession no.: NM_004421.2; NM_181870.1; NM_182779.3
• UniProt accession no.: O14640
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... DVL1(1855)

Description

General description

85 kDa

DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation. The protein acts as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 mutation is likely responsible for transformation in neuroblastomas, and the Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1 gene, thus DVL1 maybe important in these diseases as well.

Immunogen

Synthetic peptide corresponding to amino acids 557-571 of human dishevelled-1 protein.

Application

Anti-Dishevelled-1 Antibody is an antibody against Dishevelled-1 for use in WB.

Research Category
Stem Cell Research

Research Sub Category
Hematopoietic Stem Cells

Neural Stem Cells

Western blot: 1:500 using ECL. The antibody reacts with a protein of ~85kDa. Dilute antibody in 50 mM PBS, pH 7.4 containing 1.5% sodium chloride and 1% normal animal serum (same species as the host of the secondary antibody).

Optimal working dilutions must be determined by end user.

Biochem/physiol Actions

Dishevelled-1 protein (dvl-1). By Western blot the antibody shows no cross reactivity with recombinant dishevelled -2 or dishevelled -3 protein Pre-adsorption of the antibody with the immunogen peptide completely abolishes its reactivity by Western blot against dishevelled -1 protein.

Physical form

Liquid in PBS, pH 7.4, containing 0.01M sodium azide.

Preparation Note

Maintain at -20°C in undiluted aliquots (not less than 10 μL) for up to 6 months. Avoid repeated freeze/thaw cycles. The use of high quality antiserum-grade plastic or glass is recommended.

Analysis Note

Control
SK-N-SH (neuroblastoma) whole cell lysates

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable