ABS1004

Anti-phospho-NKCC1 Antibody (Thr212/Thr217)

serum, from rabbit

• Synonym(s): phospho-NKCC1 Thr212/Thr217, Solute carrier family 12 member 2, Basolateral Na-K-Cl symporter, Bumetanide-sensitive sodium-(potassium)-chloride cotransporter 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: serum
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human
• technique(s): immunofluorescence: suitable; western blot: suitable
• NCBI accession no.: NP_001037
• UniProt accession no.: P55011
• shipped in: dry ice
• target post-translational modification: phosphorylation (pThr212/pThr217)
• Gene Information: human ... SLC12A2(6558)

Description

General description

The Na-K-Cl cotransporter 1 (NKCC1) is a membrane protein coded by the SLC12A2 gene and functions in cellular sodium/potassium/chloride transport and homeostasis. It is widely distributed throughout the body and serves important functions, especially in liquid-secreting glands. Phosphorylation of Thr212/Thr217 in the intracellular N-terminal domain of NKCC1 is correlated with NKCC1 activation.

~250 kDa observed. Likely a dimer of mature form of NKCC1, NKCC1 is glycosylated differently in different cells, leading to uncharacterized band(s) in some lysates.

Immunogen

Linear peptide corresponding to human phospho-NKCC1 Thr212/Thr217.

Application

Anti-phospho-NKCC1 Thr212/Thr217 is an antibody against phospho-NKCC1 for use in Western Blotting, Immunofluorescence.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Western Blotting Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GMB43 and GBM5 cell lysate (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).
Immunofluorescence Analysis: A representative lot detected phospho-NKCC1 Thr212/Thr217 in GBM43 human tissiue (Dr. Anders Persson, UCSF) (Grim, R.P., et al. (2012). JBC. 1-31).

Biochem/physiol Actions

Specific for mature form of phosphorylated NKCC1.

Physical form

Rabbit polyclonal serum with 0.05% sodium azide.

Unpurified

Preparation Note

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Analysis Note

Evaluated by Western Blotting in GMB43 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected phospho-NKCC1 Thr212/Thr217 in 10 µg of GMB43 cell lysate.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1