Yes, the Cell Signaling Lysis Buffer (43-040) can be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K). The buffer was tested at dilutions of 1:2, 1:5, and 1:10 in Assay Buffer, with the 1:5 dilution showing comparable results to the Assay Buffer for most cytokines and the 1:10 dilution providing optimal results if the samples can tolerate that level of dilution.
MCYTMAG-70K-PX32
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay
Simultaneously analyze multiple cytokine and chemokine biomarkers with Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
MILLIPLEX® Mouse Cytokine/Chemokine Magnetic Bead Panel - Premixed 32 Plex - Immunology Multiplex Assay
1 of 2 reviewers received a sample product or took part in a promotion
Synonym(s):
Luminex® Mouse cytokine immunoassay, Millipore Mouse cytokine immunoassay, Mouse cytokine Multiplex Assay
Sign In to View Organizational & Contract Pricing
Select a Size
Pricing and availability is not currently available.
About This Item
UNSPSC Code:
12161503
eCl@ss:
32161000
NACRES:
NA.47
Skip To
Quality Level
species reactivity
mouse
manufacturer/tradename
Milliplex®
assay range
accuracy: 85-107%
standard curve range: 3.2-10,000 pg/mL
technique(s)
multiplexing: suitable
compatibility
configured for Premixed
detection method
fluorometric (Luminex xMAP)
shipped in
wet ice
1 of 4
This Item | MCYTMAG70PMX25BK | MCYTMAG70PMX32BK | PCYTMG-40K-PX23 |
|---|---|---|---|
| species reactivity mouse | species reactivity mouse | species reactivity mouse | species reactivity nonhuman primates |
| assay range accuracy: 85-107%, standard curve range: 3.2-10,000 pg/mL | assay range - | assay range - | assay range accuracy: 70-101%, linearity: 110.3% |
| manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® | manufacturer/tradename Milliplex® |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable | technique(s) multiplexing: suitable |
| compatibility configured for Premixed | compatibility configured for Premixed | compatibility configured for Premixed | compatibility configured for Premixed |
General description
The immune system is an organized complex network of biological structures and processes that protect against disease. For example, cytokine and chemokines mediate interactions between cells directly, regulating target immune cell responses. These responses result in inflammation where the immune system attempts to eradicate foreign antigens and begin the healing process. Consequently, the inflammatory process plays a key protective role in immunity. In addition, cytokine and chemokine research is essential in understanding the immune system and its multi-faceted response to most antigens, as well as disease states such as autoimmune disease, allergic reactions, sepsis, and cancer.
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
More flexible plate and plate washer options
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
To identify specific cytokines involved in any inflammatory or immune response, it might be necessary to screen panels of cytokines, often requiring some level of automation and/or high throughput. Beads can make the process of automation and high throughput screening easier with features such as walk-away washing. Advantages even outside automation include:
More flexible plate and plate washer options
- Improved performance with turbid serum/plasma samples
- Assay results equivalent to non- beads
- Automated washing avoids many problems associated with vacuum filtration washing
MILLIPLEX® Mouse Cytokine / Chemokine panel enables you to focus on the therapeutic potential of cytokines as well as the modulation of cytokine expression. Coupled with the Luminex® xMAP® platform in a bead format, you receive the advantage of ideal speed and sensitivity, allowing quantitative multiplex detection of dozens of analytes simultaneously, which can dramatically improve productivity.
Panel Type: Cytokines/Chemokines
Application
- Analytes: Eotaxin/CCL11, G-CSF, GM-CSF, IFN-γ, IL-1α, IL-1β, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-9, IL-10, IL-12 (p40), IL-12 (p70), IL-13, IL-15, IL-17, IP-10, KC, LIF, LIX, MCP-1, M-CSF, MIG, MIP-1α, MIP-1β, MIP-2, RANTES, TNF-α, VEGF
- Recommended Sample type: plasma, serum, or cell culture supernatant
- Recommended Sample dilution: 1:2 diluted plasma or serum
- Assay Run Time: Overnight or one day. For best results an overnight incubation is recommended.
- Research Category: Inflammation & Immunology
Biochem/physiol Actions
Cross Reactivty
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Packaging
96 well plate
Premixed beads for your convenience.
Preparation Note
Recommended storage for kit components is 2 - 8°C.
Other Notes
Sensitivity: Refer to kit protocol for sensitivities for individual cytokines.
Legal Information
Luminex is a registered trademark of Luminex Corp
MILLIPLEX is a registered trademark of Merck KGaA, Darmstadt, Germany
xMAP is a registered trademark of Luminex Corp
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Signal Word
Danger
Hazard Statements
Precautionary Statements
Hazard Classifications
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Skin Sens. 1 - STOT RE 2
Target Organs
Respiratory Tract
Storage Class Code
10 - Combustible liquids
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
PDSCL
Please refer to KIT Component information
PRTR
Please refer to KIT Component information
FSL
Please refer to KIT Component information
ISHL Indicated Name
Please refer to KIT Component information
ISHL Notified Names
Please refer to KIT Component information
Cartagena Act
Please refer to KIT Component information
JAN Code
キットコンポーネントの情報を参照してください
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Ulises Urzúa et al.
Biomolecules, 10(1) (2020-01-16)
In middle-aged women, the decline of ovarian follicle reserve below a critical threshold marks menopause, leading to hormonal, inflammatory, and metabolic changes linked to disease. The highest incidence and mortality of sporadic ovarian cancer (OC) occur at post-menopause, while OC
Manju Kumari et al.
The Journal of clinical investigation, 126(8), 2839-2854 (2016-07-12)
The chronic inflammatory state that accompanies obesity is a major contributor to insulin resistance and other dysfunctional adaptations in adipose tissue. Cellular and secreted factors promote the inflammatory milieu of obesity, but the transcriptional pathways that drive these processes are
Montasir Elahi et al.
Journal of Alzheimer's disease : JAD, 51(4), 1209-1224 (2016-03-01)
Recent epidemiological evidence suggests that diabetes mellitus (DM) is a risk factor for Alzheimer's disease (AD). One of the pathological hallmarks of AD is hyperphosphorylated tau protein, which forms neurofibrillary tangles. Oxidative stress and the activation of inflammatory pathways are
Anja Fuchs et al.
Journal of immunology (Baltimore, Md. : 1950), 202(10), 3020-3032 (2019-04-17)
The inflammatory response to infection or injury dramatically increases the hematopoietic demand on the bone marrow to replace effector leukocytes consumed in the inflammatory response. In the setting of infection, pathogen-associated molecular patterns induce emergency hematopoiesis, activating hematopoietic stem and
Jennifer L Johnson et al.
Neuron, 104(4), 665-679 (2019-10-06)
In humans, disruption of nonsense-mediated decay (NMD) has been associated with neurodevelopmental disorders (NDDs) such as autism spectrum disorder and intellectual disability. However, the mechanism by which deficient NMD leads to neurodevelopmental dysfunction remains unknown, preventing development of targeted therapies.
-
Can the Cell Signaling Lysis Buffer (43-040) be used in the Mouse Cytokine Milliplex kit (MCYTOMAG-70K)?
1 answer-
Helpful?
-
-
How do I enter the high, low, and expected concentrations for the kit QCs into Xponent? Since the QC range sheet only includes the high and low ranges, could you please provide the expected concentration as well?
1 answer-
A quality control range sheet is available, providing different values for quality controls. The expected average per range can be calculated by adding the high and low numbers for each marker, then dividing the total by 2.
Helpful?
-
Active Filters
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service