SCM004

ENStem-A Neural Expansion Medium

Name: ENStem-A Neural Expansion Medium
Brand: MM

The ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture & expansion of ENStem-A Human Neural Progenitor Cells.

Synonym(s):

Neural Stem Cell Media

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This item can only be ordered on the Merck Millipore website.

About This Item

UNSPSC Code:

12352207

eCl@ss:

32160801

NACRES:

NA.71

Key Documents

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Quality Level

100

form

liquid

manufacturer/tradename

Chemicon^®

technique(s)

cell culture | stem cell: suitable

input

sample type neural stem cell(s)

shipped in

dry ice

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This Item	SCM017	SCM003	SCM005
Sigma-Aldrich SCM004 ENStem-A Neural Expansion Medium	Sigma-Aldrich SCM017 ENStem-A Neuronal Differentiation Medium	Sigma-Aldrich SCM003 Neural Stem Cell Basal Medium	Sigma-Aldrich SCM005 ReNcell NSC Maintenance Media
technique(s) cell culture \| stem cell: suitable	technique(s) cell culture \| stem cell: suitable	technique(s) cell culture \| stem cell: suitable	technique(s) cell culture \| stem cell: suitable
form liquid	form liquid	form liquid	form liquid
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
Quality Level 100	Quality Level 100	Quality Level 300	Quality Level 100
manufacturer/tradename Chemicon^®	manufacturer/tradename Chemicon^®	manufacturer/tradename Chemicon^®	manufacturer/tradename Chemicon^®
input sample type neural stem cell(s)	input sample type neural stem cell(s)	input sample type neural stem cell(s)	input sample type neural stem cell(s)

General description

ENStem-A Neural Expansion Medium is a defined serum-free formulation that has been optimized for the culture and expansion of ENStem-A Human Neural Progenitor Cells. When used in conjunction with L-Glutamine (not provided) and FGF-2 (provided), the expansion medium will allow for the maintenance and proliferation of ENStem-ATM Human Neural Progenitor Cells.

Application

Cell culture protocols for use with ENStem-A Human Neural Progenitor Cells are available in the datasheet that accompanies the ENStem-A Human Neural Progenitor Expansion Kit (Cat. No. SCR055).

Physical form

ENStem-ATM Neural Expansion Medium contains neurobasal medium, B27 supplement, and LIF.

Preparation Note

ENStem-A Neural Expansion Medium, 500 mL (Part No. SCM004a)

FGF-2, 50ug, lyophilized (Part No. GF003)

ENStem-ATM Neural Expansion Medium should be stored at -20°C until ready to use. Upon thawing, fresh L-Glutamine (not provided) should be added for a final concentration of 2 mM to the expansion medium. Thawed should be stored at 2-8°C and given a 1-month expiration dating. Dispense into aliquots to avoid repeated heating prior to each use.

FGF-2 (10 μg, lyophilized) should be reconstituted with 100 μL 5 mM Tris-HCL, pH 7.6 for a final concentration of 100 μg/mL. Dispense into aliquots to avoid repeated freeze-thaw. Store at -20°C.

Analysis Note

Each lot of medium is tested for the optimal cell growth and proliferation of ENStem-ATM Human Neural Progenitor Cells.
Sterility Testing: Negative

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SCM004:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Contralaterally transplanted human embryonic stem cell-derived neural precursor cells (ENStem-A) migrate and improve brain functions in stroke-damaged rats.

Da-Jeong Chang et al.

Experimental & molecular medicine, 45, e53-e53 (2013-11-16)

The transplantation of neural precursor cells (NPCs) is known to be a promising approach to ameliorating behavioral deficits after stroke in a rodent model of middle cerebral artery occlusion (MCAo). Previous studies have shown that transplanted NPCs migrate toward the

Articles

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

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Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

Robust Differentiation of Human iPSCs into Lineage-Specific Neurons and Glial Cells Utilizing Dual-SMAD Inhibition

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Protocols

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

Dual SMAD inhibition is a well-established method to derive neural progenitor cells from both human ES and iPS cells. This protocol uses two SMAD inhibitors, Noggin and SB431542, to drive the rapid differentiation of ES/iPS cells into a highly enriched population of NPCs. Noggin acts as a BMP inhibitor and SB431542 inhibits the Lefty/Activin/TGFβ pathways by blocking the phosphorylation of ALK4, ALK5, and ALK7 receptors. In an effort to make a more defined and optimized neuronal differentiation protocol, Li and colleagues modified the original protocol to establish a completely small molecule-based differentiation method, which relies on three small molecules to inhibit GSK-3β (CHIR99021), TGFβ (SB431542), and Notch (compound E) signaling pathways, along with human LIF3. This new small molecule-based neural differentiation protocol increased neural differentiation kinetics and allowed the derivation of truly multipotent neural stem cells that respond to regional patterning cues specifying forebrain, midbrain, and hindbrain neural and glial subtypes.

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

Derivation of oligodendrocyte progenitor cells from a human neural stem cell line

ENStem-A Adherent Human Neural Progenitors: A New Source of Primary Human Neural Cells

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