WBAVDCH01
Immobilon® Block - CH (Chemiluminescent Blocker)
Chemiluminescent detection kit used for Western blotting, dot/slot blotting
Synonym(s):
Western blot blocking buffer, blocking buffer
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conjugate
peroxidase conjugate
Quality Level
packaging
pkg of 500 mL
manufacturer/tradename
Immobilon®
technique(s)
western blot: suitable
membrane area
1000 cm2 , membrane coverage
detection method
chemiluminescent
shipped in
ambient
storage temp.
15-25°C
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This Item | WBAVDFL01 | W0138 | WBKLS |
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) - | technique(s) western blot: suitable |
| detection method chemiluminescent | detection method fluorometric | detection method - | detection method chemiluminescent |
| manufacturer/tradename Immobilon® | manufacturer/tradename Immobilon® | manufacturer/tradename - | manufacturer/tradename Immobilon® |
| Quality Level 300 | Quality Level 300 | Quality Level 200 | Quality Level 200 |
| storage temp. 15-25°C | storage temp. 15-25°C | storage temp. 2-8°C | storage temp. - |
| shipped in ambient | shipped in ambient | shipped in - | shipped in ambient |
General description
Application
Packaging
Preparation Note
Analysis Note
Legal Information
Disclaimer
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Aquatic Chronic 3 - Skin Sens. 1
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
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JAN Code
WBAVDCH01:
WBAVDCH01-100ML:
Certificates of Analysis (COA)
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我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください！それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
In Western blotting, blocking of unbound membrane sites is necessary to prevent non-specific binding of the antibodies which otherwise would lead to a high background on the blot. The traditional milk blocker can work well; however, inadvertent variations in its composition can lead to irreproducible results. Such variations include the milk’s concentration, fat content, solubility, detergent quality, and numerous other factors. Bløk reagents are a family of protein-free, noisecancelling reagents that reduce background for consistent, quality results. They are available in three room temperature-stable, ready-to-use formulations for Chemiluminescence and chromogenic detection, Fluorescence detection, and Phosphoprotein immunodetection. The protein-free nature of Bløk reagents allows for membranes to be stained with chromogenic reagents, such as Ponceau S or Coomassie™ blue, after blocking or immunodetection. Bløk reagents have been tested and validated for Western blot, dot blot, and ELISA applications.
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