Everyone in our lab likes this Immobilon Crescendo ECL reagent. Signal is not too strong or too weak. just right. It's a lab staple for us. Occasionally we order the stronger version of it: FEMTO, if signal is not strong enough.
WBLUR
Immobilon® Crescendo Western HRP substrate
Western blotting, dot/slot blotting
Immobilon Crescendo Western HRP substrate, 100 mL
1 of 1 reviewers received a sample product or took part in a promotion
Synonym(s):
HRP substrate, chemiluminescent detection substrate, detection substrate
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This item can only be ordered on the Merck Millipore website.
About This Item
UNSPSC Code:
41116010
eCl@ss:
42029053
NACRES:
NB.22
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Product Name
Immobilon Crescendo Western HRP substrate, 100 mL, Western blotting, dot/slot blotting
conjugate
peroxidase conjugate
Quality Level
packaging
pkg of 100 mL
manufacturer/tradename
Luminata
technique(s)
western blot: suitable
membrane area
1000 cm2 , membrane coverage
detection method
chemiluminescent
shipped in
ambient
1 of 4
This Item | WBLUF | WBLUC | WBKLS |
|---|---|---|---|
| technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable | technique(s) western blot: suitable |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
| detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent | detection method chemiluminescent |
| shipped in ambient | shipped in ambient | shipped in ambient | shipped in ambient |
| conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate | conjugate peroxidase conjugate |
| packaging pkg of 100 mL | packaging pkg of 100 mL | packaging - | packaging pkg of 250 mL Luminol, pkg of 250 mL Peroxide solution |
General description
Premixed, ready to use chemiluminescent HRP detection reagent
Application
For use on immunoblots.
Western blotting, dot/slot blotting
Packaging
1 bottle for 1000 cm2
Preparation Note
Stable for 1 year at 4°C from date of shipment. Contains hydrogen peroxide. Use in well ventilated area.
Analysis Note
The reagent has been matched to optimize the range and sensitivity of detection using various antibodies in Western blot assays.
Legal Information
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
WGK
WGK 2
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
WBLUR0100:
WBLUR0500:
Certificates of Analysis (COA)
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Articles
A discussion of factors affecting observed vs. calculated molecular weight in gel electrophoresis and western blotting.
Protocols
後続のウェスタンブロッティングのためのサンプル調製（培養組織・細胞の効果的な溶解とタンパク質抽出）プロトコル
Protocol for sample preparation for cell lysis and efficient protein extraction from cultured tissues and cells for subsequent Western blotting.
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我々はそれを理解している。ウエスタンはしみ抜きで苦労することがある。そこで、あなたの醜いブロットを私たちと共有し、サプライズを受け取ってください！それが何であるかは言えませんが-それは驚きを台無しにしてしまうからです-ラボの創造性を掻き立てるということを除いては。
We get it. Westerns can be a pain in the blot, and we’ve certainly made our share. So, share with us your ugly blots and receive a surprise! And while we can’t tell you what it is – that would spoil the surprise - except that it will spark creativity in the lab.
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