Trypsin recombinant, Proteomics Grade

Name: Trypsin recombinant, Proteomics Grade
Brand: ROCHE
Price: 15700 JPY
Availability: InStock

recombinant enzyme expressed in Pichia pastoris

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Size/SKU	Availability	Price
4 x 25 μg	Estimated to ship onSeptember 14, 2026fromsìså¬368	¥15,700
4 x 100 μg	Estimated to ship onSeptember 14, 2026from市川市大町368	¥48,100

About This Item

EC Number:

3.4.21.4 (BRENDA, IUBMB)

NACRES:

NA.21

UNSPSC Code:

12352204

Specific activity:

≥150 units/mg protein

Assay:

95% (gel filtration)

Recombinant:

expressed in Pichia pastoris

¥15,700

Estimated to ship onSeptember 14, 2026Details

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Properties

recombinant

expressed in Pichia pastoris

assay

95% (gel filtration)

form

lyophilized

specific activity

≥150 units/mg protein

packaging

pkg of 4 × 100 μg (03708969001), pkg of 4 × 25 μg (03708985001)

manufacturer/tradename

Roche

storage temp.

−20°C

Description

General description

This trypsin preparation is generated from the recombinant Pichia pastoris strain and is, thus, free of chymotrypsin. Trypsin recombinant, Proteomics Grade, exhibits high specific activity.

Application

Trypsin recombinant, Proteomics Grade, is used to specifically digest proteins separated by 2D gel electrophoresis or liquid chromatographic methods during sample preparation for mass spectrometry.

Biochem/physiol Actions

Serine endopeptidase, hydrolyzing specifically proteins and peptides at the carboxy side of the basic amino acids Arg and Lys. Amide and ester bonds of Arg and Lys are also cleaved.

Preparation Note

Stabilizers: Ca²⁺ in mM concentration range
Inhibitors: TLCK, DFP, PMSF, leupeptin, soybean trypsin inhibitor, trypsin inhibitor from hen egg, aprotinin, α₂-macroglobulin, α₁-antitrypsin, APMSF, and antipain.
Storage conditions (working solution): -15 to -25°C
The reconstituted solution is stable for 1 month at -15 to -25°C. Avoid repeated freezing/thawing!

Following reconstitution in 10 mM HCl, Trypsin, recombinant, proteomics grade is stable at -15 to -25 °C for up to one month. After first reconstitution the product must be stored in appropriate aliquots to avoid repeated freezing and thawing.