This product is offered in a set of 6 vials, each containing 10 mL of an approximately 0.5M solution of dibutylamine acetate. When diluted to the recommended 1L volume, the resulting concentration is approximately 0.005M. The Certificate of Analysis reports the molarity of each lot of the concentrate, which may range from 0.48M - 0.52M. Please see the link below to review a sample or lot specific Certificate:
https://www.sigmaaldrich.com/product/sial/73345#product-documentation
73345
Dibutylammonium acetate solution
0.5 M in H2O, LiChropur™, suitable for ion pair chromatography
Synonym(s):
Dibutylamine acetate Concentrate
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About This Item
Linear Formula:
(CH3CH2CH2CH2)2NH · CH3COOH
CAS Number:
Molecular Weight:
189.30
MDL number:
UNSPSC Code:
12161700
PubChem Substance ID:
NACRES:
NB.21
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form
solution
Quality Level
quality
LiChropur™
packaging
pkg of 25 ML Volume containing 10 ML of product
concentration
0.5 M in H2O
technique(s)
LC/MS: suitable
ion pair chromatography: suitable
refractive index
n20/D 1.348
SMILES string
CC(O)=O.CCCCNCCCC
InChI
1S/C8H19N.C2H4O2/c1-3-5-7-9-8-6-4-2;1-2(3)4/h9H,3-8H2,1-2H3;1H3,(H,3,4)
InChI key
MQFIKAWTCOXAAY-UHFFFAOYSA-N
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This Item | 93645 | 49540 | 625718 |
|---|---|---|---|
| technique(s) LC/MS: suitable, ion pair chromatography: suitable | technique(s) ion pair chromatography: suitable | technique(s) ion pair chromatography: suitable | technique(s) - |
| concentration 0.5 M in H2O | concentration 0.45-0.55 M, 0.5 M in H2O | concentration 0.5 M in H2O | concentration - |
| Quality Level 100 | Quality Level 100 | Quality Level 100 | Quality Level 200 |
| form solution | form liquid | form liquid | form solution |
| quality LiChropur™ | quality LiChropur™ | quality LiChropur™ | quality - |
| packaging pkg of 25 ML Volume containing 10 ML of product | packaging - | packaging - | packaging - |
General description
Dibutylammonium acetate is an ion-pairing agent with quaternary ammonium ions. It generally helps in ionization of carbohydrate fragments providing a volatile solvent for the mass spectrometric technique.
Application
It has been used as buffer in mobile phase for separation and quantifying intracellular nucleotides from different microorganisms using ion pair reversed phase liquid chromatography electrospray ionization isotope dilution tandem mass spectrometry (IP–LC–ESI–ID–MS/MS) method.[1]
Packaging
Clear borosilicate glass, high hydrolytic resistance (Type I)
concentrate in ampules for 6x1 l 0.005 M ready-to-use solution
Legal Information
LiChropur is a trademark of Merck KGaA, Darmstadt, Germany
related product
Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
73345-6EA:
73345-BULK:
73345-VAR:
73345-1EA:
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Simultaneous quantification of free nucleotides in complex biological samples using ion pair reversed phase liquid chromatography isotope dilution tandem mass spectrometry.
Seifar, Reza M., et al.
Analytical Biochemistry, 388.2, 213-219 (2009)
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Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 967, 127-133 (2014-08-05)
Targeted metabolite profiling has aided in the understanding of a variety of biological processes in the malaria parasite as well as in drug discovery. A fast and sensitive analytical method, based on ion-pairing reversed phase ultra-high performance liquid chromatography tandem
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