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Sigma-Aldrich

DL-Dithiothreitol solution

BioUltra, for molecular biology, ~1 M in H2O

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Synonym(s):
Cleland′s reagent, (±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, (±)-threo-1,4-Dimercapto-2,3-butanediol solution, DTT
Empirical Formula (Hill Notation):
C4H10O2S2
CAS Number:
Molecular Weight:
154.25
Beilstein:
1719757
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

Assay

900-1100 mmol/L (Titration molarity)

form

liquid

reaction suitability

reagent type: reductant

concentration

~1 M in H2O

impurities

DNases, none detected
RNases, none detected
phosphatases, none detected
proteases, none detected

pH

2.7-5.0

density

1.04 g/mL at 20 °C

absorption

cut-off at 330 nm

suitability

in accordance for filter test
suitable for molecular biology

storage temp.

2-8°C

SMILES string

O[C@H](CS)[C@H](O)CS

InChI

1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1

InChI key

VHJLVAABSRFDPM-QWWZWVQMSA-N

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General description

Dithiothreitol (DTT), a thiol group protectant, is an excellent reagent for preventing the oxidation of SH groups in proteins. DTT solution is a potent and versatile disulfide-reducing agent. It reduces a disulfide bond by forming a six-membrane ring and initiating a thiol-disulfide interchange.

Application

DL-dithiothreitol solution has been used:

  • in the treatment of intestinal explants
  • in homogenate preparation of gill cells for enzymatic assays
  • in protein electrophoresis for western blotting
  • in transglutaminase activity
  • in sample buffer for SDS-PAGE
  • in Western Blot, as a lysis buffer component for single-nucleus RNA-sequencing
  • as a component of protein extraction buffer for cell lysis

Biochem/physiol Actions

DL-dithiothreitol (DTT) is used in a chemical debonding technique, that helps to remove bacteria from biofilms. It can change the structural stability of the GABA(B) (gamma-aminobutyric acid) receptor by disabling the disulfide bonds between four cysteine residues positioned in the GABA(B1(a)) receptor structure.Dithiothreitol (DTT) is broadly utilized in synthetic peptide synthesis and biochemical arrangements of thiol proteins. It can also be used to study protein chemistry topics like enzyme activity and protein folding. DTT explicitly controls the thiol-disulfide exchange response to completely reduce the intra-or between sub-atomic disulfide bonds in biomolecules. Thiols and the cyclic disulfide of DTT are the products of this reaction.

Features and Benefits

  • BioUltra grade - 1M DTT solution, suitable for molecular biology
  • Free from DNases, RNases, phosphatases, and proteases

Other Notes

For additional information on our range of Biochemicals, please complete this form.

Pictograms

Corrosion

Signal Word

Danger

Hazard Statements

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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