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G0251

β-Glucuronidase from bovine liver

Type B-1, ≥1,000,000 units/g solid

Synonym(s):

Bovine Liver beta-Glucuronidase, β-D-Glucuronide glucuronosohydrolase

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About This Item

CAS Number:
9001-45-0
EC Number:
3.2.1.31 (BRENDA, IUBMB)
EC Number:
232-606-8
MDL number:
MFCD00130629
UNSPSC Code:
12352204
NACRES:
NA.54

Key Documents

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type

Type B-1

form

solid

specific activity

≥1,000,000 units/g solid

storage temp.

−20°C

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1 of 4

This Item
G0376G0799G7017
β-Glucuronidase from bovine liver Type B-1, ≥1,000,000 units/g solid

Sigma-Aldrich

G0251

β-Glucuronidase from bovine liver

β-Glucuronidase from bovine liver Type B-3, ≥2,000,000 units/g solid

Sigma-Aldrich

G0376

β-Glucuronidase from bovine liver

β-Glucuronidase from Escherichia coli >20,000,000 units/g protein, recombinant, expressed in E. coli, aqueous glycerol solution

Sigma-Aldrich

G0799

β-Glucuronidase from Escherichia coli

β-Glucuronidase from Helix pomatia Type HP-2, aqueous solution, ≥100,000 units/mL

Sigma-Aldrich

G7017

β-Glucuronidase from Helix pomatia

specific activity

≥1,000,000 units/g solid

specific activity

≥2,000,000 units/g solid

specific activity

>20,000,000 units/g protein

specific activity

≥100,000 units/mL

form

solid

form

solid

form

aqueous glycerol solution

form

aqueous solution

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

300

type

Type B-1

type

Type B-3

type

-

type

Type HP-2

General description

β-Glucuronidase (GUSB) is a lysosomal enzyme,[1] found in animals, plants and bacteria.[2] This exoglycosidase shows high activity in spleen, liver and kidney. Bovine liver β-glucuronidase (BLG) is homotetramer with a molecular weight of 290 kDa.[2]

Application

β-Glucuronidase from bovine liver has been used in β-glucuronidase inhibition assay to study the β-glucuronidase inhibitory effect of extracts and isolated actives from Phyllanthus amarus.[3] It has also been used to perform deglucuronidation.[4]
β-glucuronidase is used as a reporter gene in GUS assays to monitor gene expression.

Biochem/physiol Actions

β-Glucuronidase (GUSB) deficiency can result in mucopolysaccharidosis type VII (MPSVII), which leads to store lysosome in the brain.[1] Integration of β-glucuronides of steroid hormones into tissues can be done with the help of GUSB.[2]
β-glucuronidase catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.

Other Notes

One Sigma or modified Fishman unit will liberate 1.0 μg of phenolphthalein from phenolphthalein glucuronide per hr at 37 °C at pH 5.0 (30 min assay).

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

G0251-CP:
G0251-100KU:
G0251-VAR:
G0251-5MU:
G0251-500KU:
G0251-1MU:
G0251-BULK:

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Certificates of Analysis (COA)

Lot/Batch Number
0000483424
0000483407
0000483407
0000483424
0000479349

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A D Fraser
Journal of analytical toxicology, 11(6), 263-266 (1987-11-01)
Alprazolam (Xanax) and triazolam (Halcion) are relatively new triazolobenzodiazepines that are anxiolytic and hypnotic. This study assesses the reactivity of these drugs and their major metabolites in the EMIT d.a.u. benzodiazepine metabolite assay. Analytical standards of drugs and metabolites and
Chloé Hugbart et al.
Toxicology in vitro : an international journal published in association with BIBRA, 66, 104842-104842 (2020-04-14)
Ethanol is the most frequently psychoactive substance used in the world, leading to major public health problems with several millions of deaths attributed to alcohol consumption each year. Metabolism of ethanol occurs mainly in the liver via the predominant oxidative
Determination of furosemide with its acyl glucuronide in human plasma and urine by means of direct gradient high-performance liquid chromatographic analysis with fluorescence detection Preliminary pharmacokinetics and effect of probenecid
Vree TB, et al.
Journal of Chromatography. B, Biomedical Sciences and Applications, 655(1), 53-62 (1994)
Uzma Salar et al.
European journal of medicinal chemistry, 125, 1289-1299 (2016-11-26)
Current study is based on the biology-oriented drug synthesis (BIODS) of 2-(2-methyl-5-nitro-1H-imidazol-1-yl)ethyl aryl carboxylate derivatives 1-26, by treating metronidazole with different aryl and hetero-aryl carboxylic acids in the presence of 1,1'-carbonyl diimidazole (CDI) as a coupling agent. Structures of all
P Jakobsen et al.
The Journal of pharmacy and pharmacology, 33(2), 89-92 (1981-02-01)
Sulphinpyrazone is an antiplatelet in vivo and in vitro. Two active metabolites, a sulphide (S) and a hydroxylated sulphide (S-OH) have been identified in rabbit and human plasma and a selective and sensitive g.c.-m.s.-method for quantitative determination of the sulphide
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Protocols

UHPLC/MS for Drug Detection in Urine

Optimize β-glucuronidase hydrolysis for glucuronide metabolite analysis considering factors like time, temperature, pH, and enzyme concentration.

ß-Glucuronidase Enzymatic Assay

Enzymatic assay protocol for β-Glucuronidase from Helix Pomatia and Bovine Liver aids laboratory personnel in following procedures.

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