The product is stable for nearly 3 years when stored at room temperature. There is no specific requirement to store L2542 at 2-8°C. However, if the container is opened and closed, there is a possibility of contamination through routine use.
L3522
LB Broth (Miller)
Highly-referenced nutrient-rich microbial growth powder medium, suitable for regular E.coli culture
Synonym(s):
Luria Broth, Miller’s LB Broth
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250 G
¥4,840
1 KG
¥22,720
About This Item
UNSPSC Code:
41106200
NACRES:
NA.85
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grade
Molecular Biology
Quality Level
description
Miller′s Modification, 10 g/L NaCl
sterility
non-sterile
form
powder
composition
NaCl, 10 g/L
Tryptone, 10 g/L
Yeast Extract, 5 g/L
pH
6.8-7.2(2.5% solution)
application(s)
food and beverages
storage temp.
room temp
suitability
nonselective for Escherichia coli
nonselective for coliforms
1 of 4
This Item | L2542 | L3147 | L3022 |
|---|---|---|---|
| suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms | suitability nonselective for Escherichia coli, nonselective for coliforms |
| form powder | form liquid | form powder | form powder |
| composition NaCl, 10 g/L , Yeast Extract, 5 g/L , Tryptone, 10 g/L | composition - | composition Agar, 15 g/L , NaCl, 10 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L | composition NaCl, 5 g/L , Tryptone, 10 g/L , Yeast Extract, 5 g/L |
| sterility non-sterile | sterility sterile; 0.2 μm filtered | sterility non-sterile | sterility non-sterile |
| application(s) food and beverages | application(s) microbiology | application(s) food and beverages | application(s) food and beverages |
| pH 6.8-7.2(2.5% solution) | pH 6.5-7.2 | pH 6.8-7.2(4% solution) | pH 6.8-7.2(2% solution) |
General description
Miller′s LB is a highly-referenced microbial growth medium used for the cultivation of E. coli. This nutrient-rich microbial broth contains peptides, amino acids, water-soluble vitamins, and carbohydrates.
Application
LB Broth (Miller) has been used as a culture medium for E coli,[1] Pseudomonas putida KT2440,[2] Vibrio parahaemolyticus,[3] and soil samples for the isolation of Bacillus spp.[4]
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Suitable for non-selective cultivation of E. coli strains for cloning, DNA plasmid production and production of recombinant proteins. Also suitable for selective cultivation when appropriate antibiotics are added.
Biochem/physiol Actions
Luria Broth is used for maintenance and propagation of Escherichia coli. The E. coli grow faster in Luria Broth because the tryptone and yeast supply essential growth factors that the E. coli would otherwise have to synthesize. Luria Broth also contains essential electrolytes for transport and osmotic balance, due to the NaCl component[5].
Features and Benefits
Miller′s LB powder provides:
- Easy scale-up using larger package sizes
- A budget-friendly alternative to liquid
- Standard formulation
Preparation Note
1. Suspend 25 g in 1 L of distilled water.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
2. Autoclave for 15 minutes at 121 °C.
To prepare the medium of Luria, Adams and Ting (also known as LC broth: Aseptically add 25 ml of sterile 0.1 M calcium chloride after autoclaving.
Stir to suspend 25g powder in 1L water. Autoclave for 15 minutes at 121C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).
related product
Product No.
Description
Pricing
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Regulatory Listings
Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.
JAN Code
L3522-EW:
L3522-VAR:
L3522-BULK:
L3522-1KG-PW:
L3522-250G-PW:
L3522-250G:4548173201122
L3522-1KG:4548173201115
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Ikhlaas M Kasli et al.
AMB Express, 9(1), 5-5 (2019-01-09)
Production of recombinant proteins such as antibody fragments in the periplasm of the bacterium Escherichia coli has a number of advantages, including the ability to form disulphide bonds, aiding correct folding, and the relative ease of release and subsequent capture
Single-cell bacteria growth monitoring by automated DEP-facilitated image analysis
Peitz I and van Leeuwen R
Lab on a chip, 10(21), 2944-2951 (2010)
Demeng Tan et al.
Microorganisms, 8(3) (2020-03-12)
Phage therapy is a potential and promising avenue for controlling the emergence and spread of multidrug-resistant (MDR) Klebsiella pneumoniae, however, the rapid development of anti-phage resistance has been identified as an obstacle to the development of phage therapy. Little is
Selecting lipopeptide-producing, Fusarium-suppressing Bacillus spp.: Metabolomic and genomic probing of Bacillus velezensis NWUMFkBS10. 5
Adeniji A, et al.
MicrobiologyOpen, e742-e742 (2018)
Atlas, R. M., L.C. Parks
Handbook of Microbiological Media (1993)
Articles
SeqPlex™-I WTA kit amplifies RNA for NGS, enabling genomic studies from limited samples.
遺伝子工学により、研究のための遺伝子組換えタンパク質の大規模な発現・単離が可能になります。
Genetic engineering enables large-scale expression and isolation of recombinant proteins for research purposes.
Protocols
General protocols for growth of competent cells in microbial medium.
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What is the recommended duration for using this item after autoclaving, and can it be stored at 4 degrees Celsius for extended or long-term use?
1 answer-
Helpful?
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Will adding magnesium to the culture medium increase cell density?
1 answer-
In microbial broth formulations that do not already contain magnesium, the addition of 10-20 mM MgCl2 or MgSO4 may increase cell densities. You may need to also increase the shaking speed of the incubator.
Helpful?
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What is the difference among the LB - Miller products?
1 answer-
LB - Miller is available in many types to suit your needs. The different product formats include powder and liquid form. The powder form is also available with agar for easy LB-agar plate preparation.L2542 (LB Miller liquid)L3522 (LB Miller powder)L3147 (LB Miller powder with agar)
Helpful?
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What are the differences among the Luria, Lennox and Miller LB formulations?
1 answer-
LB, (originally termed lysogeny broth) was initially composed of tryptone, yeast extract, NaCl and glucose. Soon after, the glucose was omitted (Miller's version), and later the NaCl content lowered by half (Lennox's version). For some applications, even lower salt is required (Luria's low salt version).
Helpful?
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Which bacterial culture medium is the best choice for my application?
1 answer-
Each of the broths will likely grow E. coli very well, but there are still general guidelines for choosing a broth when you are working without a protocol. Generally:LB - Miller and LB - Lennox are used for E. coli growth and maintanence, DNA plasmid production and protein production. The Lennox formulation has a lower salt content required for some salt-sensitive selection antibiotics.LB - Luria low salt is used for special applications where the E. coli growth or other constraints require the lowest possible salt content.Terrific Broth is used for higher yield protein production and high yield DNA plasmid production, because of the faster growth of the E. coli in this medium.SOB is used for protein production, DNA plasmid production and the generation of high-efficiency competent cells.SOC is used for initial growth of competent cells and the transformation procedure.
Helpful?
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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