Name: Lysozyme from chicken egg white
Brand: SIGMA
Price: 38080 JPY

Product Name

Lysozyme from chicken egg white, aseptically filled

sterility

aseptically filled

form

powder

enzyme activity

≥40000 units/mg protein

mol wt

single-chain 14.3 kDa

UniProt accession no.

P00698

storage temp.

−20°C

Quality Level

300

Gene Information

chicken ... LYZ(396218)

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Cell Lysis Buffers & Protein Extraction Reagents

Protein Sample Prep

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Show Differences

1 of 4

This Item	62970	L6876	L4631
Sigma-Aldrich L7773 Lysozyme from chicken egg white	Sigma-Aldrich 62970 Lysozyme from chicken egg white	Sigma-Aldrich L6876 Lysozyme from chicken egg white	Sigma-Aldrich L4631 Lysozyme from chicken egg white
Gene Information chicken ... LYZ(396218)	Gene Information chicken ... LYZ(396218)	Gene Information chicken ... LYZ(396218)	Gene Information chicken ... LYZ(396218)
form powder	form powder	form powder or granules	form powder
mol wt single-chain 14.3 kDa	mol wt single-chain 14.3 kDa, M_r ~14600	mol wt single-chain 14.3 kDa	mol wt single-chain 14.3 kDa
storage temp. −20°C	storage temp. 2-8°C	storage temp. room temp	storage temp. 2-8°C
UniProt accession no. P00698	UniProt accession no. P00698	UniProt accession no. P00698	UniProt accession no. P00698
sterility aseptically filled	sterility -	sterility -	sterility -

Application

Enzyme breaks down the cell walls of bacteria; used to prepare spheroplasts.

Biochem/physiol Actions

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan. Gram-negative bacteria are less susceptible due to the presence of an outer membrane and a lower proportion of peptidoglycan. However, these cells may be hydrolyzed in the presence of EDTA that chelates metal ions in the outer bacterial membrane.

The enzyme is active over a broad pH range (6.0 to 9.0). At pH 6.2, maximal activity is observed over a wider range of ionic strengths (0.02 to 0.100 M) than at pH 9.2 (0.01 to 0.06 M).

Lysozyme hydrolyzes β(1→4) linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrin. Gram-positive cells are quite susceptible to this hydrolysis as their cell walls have a high proportion of peptidoglycan.

Other Notes

One unit will lyse 0.6 μg of Micrococcus lysodeikticus per minute by turbidimetric detection at 600 nm when suspended in buffer at pH 6.2 at 25 °C.

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Preparation Note

Prepared from L6876

used together

Product No.

Description

Pricing

M5639

4-Methylumbelliferyl β-D-N,N′,N′′-triacetylchitotrioside, fluorogenic glycanase substrate

N8638

4-Nitrophenyl β-D-N,N′,N′′-triacetylchitotriose, chitinase and lysozyme substrate

pictograms

GHS08

signalword

Danger

hcodes

H334

pcodes

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number

0000501200

0000326833

0000315433

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A lysozyme-like protein in Brucella abortus is involved in the early stages of intracellular replication.

Mariela G Del Giudice et al.

Infection and immunity, 81(3), 956-964 (2013-01-16)

Secretion of proteins in Gram-negative bacteria is a high-energy-consuming process that requires translocation across two membranes and a periplasmic space composed of a mesh-like layer, the peptidoglycan. To achieve this, bacteria have evolved complex secretion systems that cross these barriers

[Pharmacological correction of nonspecific resistance and production of proinflammatory cytokines during chronic intoxication with organophosphorus compound VX].

P F Zabrodskiĭ et al.

Eksperimental'naia i klinicheskaia farmakologiia, 75(11), 19-21 (2013-01-18)

It is established in experiments on noninbred rats that the use of imunofan (20 mg/kg daily) and polyoxidonium (150 mg/kg daily) for 7 days on the background of chronic intoxication with organophosphorus agent VX (0.01 LD50, single daily treatment for

Cell wall elongation mode in Gram-negative bacteria is determined by peptidoglycan architecture.

Robert D Turner et al.

Nature communications, 4, 1496-1496 (2013-02-21)

Cellular integrity and morphology of most bacteria is maintained by cell wall peptidoglycan, the target of antibiotics essential in modern healthcare. It consists of glycan strands, cross-linked by peptides, whose arrangement determines cell shape, prevents lysis due to turgor pressure

Investigation on the interaction between cyclophosphamide and lysozyme in the presence of three different kind of cyclodextrins: determination of the binding mechanism by spectroscopic and molecular modeling techniques.

Mahdieh Mansouri et al.

Molecules (Basel, Switzerland), 18(1), 789-813 (2013-01-25)

The interactions between cyclophosphamide (CYC) and lysozyme (LYZ) in the presence of different cyclodextrins (CDs) were investigated by UV absorption, fluorescence spectroscopy, circular dichroism (CD), and molecular modeling techniques under imitated physiological conditions. The UV absorption results showed the formation

A universal, high recovery assay for protein quantitation through temperature programmed liquid chromatography (TPLC).

Dennis J Orton et al.

Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 921-922, 75-80 (2013-02-26)

As an alternative to direct UV absorbance measurements, estimation of total protein concentration is typically conducted through colorimetric reagent assays. However, for protein-limited applications, the proportion of the sample sacrificed to the assay becomes increasingly significant. This work demonstrates a

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Protocols

Enzymatic Assay of Lysozyme (EC 3.2.1.17)

This enzymatic rate determination may be used for Lysozyme products. It is not to be used to assay recombinant or insoluble Lysozyme on agarose.

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Lysozyme from chicken egg white

aseptically filled

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L7773-50MG

¥38,080

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