ABN2252

Anti-Neuroligin-4

• 別名: Neuroligin Y
• UNSPSCコード: 12352203
• NACRES: NA.41

特性

• 由来生物: rabbit
• 品質水準: 200
• 結合体: unconjugated
• 抗体製品の状態: purified antibody
• 抗体製品タイプ: primary antibodies
• クローン: polyclonal
• 交差性: human
• 包装: antibody small pack of 100
• テクニック: immunofluorescence: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• アイソタイプ: IgG
• エピトープ配列: C-terminal cytoplasmic domain
• タンパク質IDアクセッション番号: NP_055708
• UniProtアクセッション番号: Q8NFZ3
• ターゲットの翻訳後修飾: unmodified
• 遺伝子情報: human ... NLGN4Y(22829)

詳細

詳細

Neuroligin-4 (UniProt: Q8NFZ3; also known as Neuroligin Y) is encoded by the NLGN4Y (also known as KIAA0951) gene (Gene ID: 22829) in human. Neuroligins are postsynaptic cell adhesion molecules that have crucial roles in synapse maturation and function. In humans, NLGN4X is located on the X chromosome and the NLGN4Y is localized on the Y chromosome and together they form an X-Y pair. They share about 97% amino acid identity; however, they are differentially regulated. Although several mutations in NLGN4X have been linked to autism, only one mutation has been identified in NLGN4Y gene. Despite their high degree of sequence identity, NLGN4Y exhibits severe deficit in trafficking to cell surface and related synaptic effects. This has been attributed to serine 93 in NLGN4Y. A substitution of serine to proline at residue 93 is shown to rescue it and increase its surface trafficking. Neuroligin-4 is a single-pass type I, homodimeric, membrane glycoprotein that is synthesized with a signal peptide (aa 1-43), which is subsequently cleaved off to produce the mature form that contains an extracellular domain (aa 44-676), a transmembrane domain (aa 677-697), and a cytoplasmic domain (aa 698-816). Its expression is observed in fetal and adult brain, prostate, and testis. It serves as a neuronal cell surface protein that is involved in cell-cell interactions. (Ref.: Nguyen, TA., et al. (2020). Neuron 106(5); 759-768; Marro, SG., et al. (2019). Neuron. 103(4); 617-626).

免疫原

KLH-conjugated linear peptide corresponding to 16 amino acids from the C-terminal, cytoplasmic domain of human Neuroligin-4.

アプリケーション

Quality Control Testing

Evaluated by Western Blotting in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Neuroligin-4 in lysate from HEK293T cells overexpressing Human Neuroligin-4.

Tested Applications

Immunofluorescence Analysis: A representative lot detected Neuroligin-4 in Immunofluorescence applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Western Blotting Analysis: A representative lot detected Neuroligin-4 in Western Blotting applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Immunoprecipitation Analysis: A representative lot immunoprecipitated Neuroligin-4 in Immunoprecipitation applications (Nguyen, T.A., et. al. (2020). Neuron. 106(5):759-768).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

生物化学的／生理学的作用

This rabbit polyclonal antibody detects Neuroligin-4. It targets an epitope within 16 amino acids from the C-terminal, cytoplasmic domain.

物理的形状

Purified rabbit polyclonal antibody in buffer containing PBS, pH 7.4, without azide.

調製ノート

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全性情報

• 保管分類コード: 12 - Non Combustible Liquids
• WGK: WGK 2
• 引火点(°F): Not applicable
• 引火点(℃): Not applicable