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Target-responsive "sweet" hydrogel with glucometer readout for portable and quantitative detection of non-glucose targets.

Journal of the American Chemical Society (2013-01-24)
Ling Yan, Zhi Zhu, Yuan Zou, Yishun Huang, Dewen Liu, Shasha Jia, Dunming Xu, Min Wu, Yu Zhou, Shuang Zhou, Chaoyong James Yang
ABSTRACT

Portable devices with the advantages of rapid, on-site, user-friendly, and cost-effective assessment are widely applied in daily life. However, only a limited number of quantitative portable devices are commercially available, among which the personal glucose meter (PGM) is the most successful example and has been the most widely used. However, PGMs can detect only blood glucose as the unique target. Here we describe a novel design that combines a glucoamylase-trapped aptamer-cross-linked hydrogel with a PGM for portable and quantitative detection of non-glucose targets. Upon target introduction, the hydrogel collapses to release glucoamylase, which catalyzes the hydrolysis of amylose to produce a large amount of glucose for quantitative readout by the PGM. With the advantages of low cost, rapidity, portability, and ease of use, the method reported here has the potential to be used by the public for portable and quantitative detection of a wide range of non-glucose targets.

MATERIALS
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Sigma-Aldrich
アミロース ジャガイモ由来, used as amylase substrate