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  • A novel method to derive and expand mice neural stem cells efficiently without neuro-sphere formation.

A novel method to derive and expand mice neural stem cells efficiently without neuro-sphere formation.

International journal of clinical and experimental medicine (2015-11-10)
Zhi-Zhao Ma, Lin Fan, Jun-Ling Huang, Xiao-Jing Pan
要旨

Neural stem cells (NSCs) are multi-potent stem cells able to self-renew and generate immature and differentiated cell populations by asymmetric division. The NSCs are of considerable interest for cell replacement in neuro-degenerative diseases. NSCs are usually identified and expanded by their ability to generate free-floating aggregates termed neurospheres. However, neurospheres are not a pure population of NSCs with as little as 1% population in primary spheres. Neurospheres also contain neurons, astrocytes and oligodendrocytes. The heterogeneity of these cells may hinder their repopulation potential when used in cell transplantation. Furthermore, to obtain 1 million NSCs by the neurosphere protocol usually takes one month, which is inconvenient for future clinical trials. In this study, we tried to derive the NSCs from mice embryo neuroepithelium without neurosphere formation. Three different protocols were compared. We generated a direct and efficient NSCs generation, expanding and freezing protocol. This protocol can provide sufficient amount of the NSCs from first a few passages for cell transplantation.

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Sigma-Aldrich
Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1), ascites fluid, clone TU-20 (Similar to TUJ1), Chemicon®
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抗GFAP マウス宿主抗体, purified immunoglobulin, buffered aqueous solution
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Anti-Synapsin, N-Terminal antibody produced in rabbit, affinity isolated antibody

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