- Analyses in zebrafish embryos reveal that nanotoxicity profiles are dependent on surface-functionalization controlled penetrance of biological membranes.
Analyses in zebrafish embryos reveal that nanotoxicity profiles are dependent on surface-functionalization controlled penetrance of biological membranes.
Scientific reports (2017-08-23)
Ilkka Paatero, Eudald Casals, Rasmus Niemi, Ezgi Özliseli, Jessica M Rosenholm, Cecilia Sahlgren
PMID28827674
要旨
Mesoporous silica nanoparticles (MSNs) are extensively explored as drug delivery systems, but in depth understanding of design-toxicity relationships is still scarce. We used zebrafish (Danio rerio) embryos to study toxicity profiles of differently surface functionalized MSNs. Embryos with the chorion membrane intact, or dechoroniated embryos, were incubated or microinjected with amino (NH
材料
製品番号
ブランド
製品内容
Sigma-Aldrich
L-グルタミン 溶液, 200 mM, solution, sterile-filtered, BioXtra, suitable for cell culture
Sigma-Aldrich
MEM非必須アミノ酸溶液 (100X), without L-glutamine, liquid, sterile-filtered, BioReagent, suitable for cell culture
Sigma-Aldrich
ペニシリン-ストレプトマイシン, with 10,000 units penicillin and 10 mg streptomycin per mL in 0.9% NaCl, 0.1 μm filtered, BioReagent, suitable for cell culture
SAFC
ダルベッコ改変イーグル培地(高グルコース), HEPES modification, With 4500 mg/L glucose, 25 mM HEPES, and sodium bicarbonate, without L-glutamine and sodium pyruvate, liquid, sterile-filtered, suitable for cell culture