77607

Phenol

BioUltra, Molecular Biology, TE-saturated, ~73% (T)

• Synonym(s): Hydroxybenzene
• Linear Formula: C₆H₅OH
• CAS Number: 108-95-2
• Molecular Weight: 94.11
• UNSPSC Code: 12352104
• NACRES: NA.25
• PubChem Substance ID: 57652703
• EC Number: 203-632-7
• Beilstein/REAXYS Number: 969616
• MDL number: MFCD00002143
• Grade: Molecular Biology
• Assay: ~73% (T)
• Bp: 182 °C (lit.)
• Vapor pressure: 0.09 psi ( 55 °C); 0.36 mmHg ( 20 °C)

Properties

• Product Name: Phenol, BioUltra, Molecular Biology, TE-saturated, ~73% (T)
• InChI key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N
• InChI: 1S/C6H6O/c7-6-4-2-1-3-5-6/h1-5,7H
• SMILES string: Oc1ccccc1
• grade: Molecular Biology
• vapor density: 3.24 (vs air)
• vapor pressure: 0.09 psi ( 55 °C); 0.36 mmHg ( 20 °C)
• product line: BioUltra
• assay: ~73% (T)
• form: liquid
• autoignition temp.: 1319 °F
• quality: TE-saturated
• contains: ~0.1% 8-hydroxyquinoline as preservative
• expl. lim.: 8.6 %
• impurities: DNases, none detected; RNases, none detected; insoluble matter, passes filter test; phosphatases, none detected; proteases, none detected
• refractive index: n20/D 1.48
• pH: ~7.8(aqueous phase)
• bp: 182 °C (lit.)
• mp: 40-42 °C (lit.)
• density: 1.071 g/mL at 25 °C (lit.)
• anion traces: sulfate (SO₄^2-): ≤50 mg/kg
• cation traces: Al: ≤5 mg/kg; As: ≤0.1 mg/kg; Ba: ≤5 mg/kg; Bi: ≤5 mg/kg; Ca: ≤10 mg/kg; Cd: ≤5 mg/kg; Co: ≤5 mg/kg; Cr: ≤5 mg/kg; Cu: ≤5 mg/kg; Fe: ≤5 mg/kg; K: ≤50 mg/kg; Li: ≤5 mg/kg; Mg: ≤5 mg/kg; Mn: ≤5 mg/kg; Mo: ≤5 mg/kg; Na: ≤50 mg/kg; Ni: ≤5 mg/kg; Pb: ≤5 mg/kg; Sr: ≤5 mg/kg; Zn: ≤5 mg/kg
• suitability: suitable for molecular biology
• storage temp.: 2-8°C
• Quality Level: 100
• Gene Information: human ... GABRA1(2554)

Description

General description

Phenol, also known as hydroxybenzene, is an aromatic compound produced both naturally and synthetically.[1] It acts as a disinfectant and is highly toxic. Exposure to phenol through inhalation, cutaneous adsorption, or oral ingestion causes denaturation and precipitation of cellular protein, which in turn leads to poisoning.[2]

saturated with 10 mM TRIS buffer pH 8.0 and 1 mM EDTA

Other Notes

RNA fingerprinting - preparation of the E. coli tRNA carrier[3]

Application

Phenol (TE-saturated) has been used in the phenol-chloroform extraction procedure for plasmid DNA isolation.[4] It has also been used to extract the aqueous phase during DNA linearization and mRNA synthesis.[5]

Disclaimer

according to temperature, a small quantity of an overlaid aqueous phase may form

Safety Information

• pictograms: GHS06,GHS08,GHS05,GHS09
• signalword: Danger
• hcodes: H301 + H311 + H331,H314,H341,H373,H411
• pcodes: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Muta. 2 - Skin Corr. 1B - STOT RE 2
• target_organs: Nervous system,Kidney,Liver,Skin
• Storage Class: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• wgk: WGK 2
• flash_point_f: 174.2 °F - closed cup
• flash_point_c: 79 °C - closed cup
• ppe: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter