To prepare a laminin solution, we recommend that the L6274 be thawed at 2-8°C to avoid the formation of a gel. This can be further diluted in a balanced salt solution. If the product has been thawed at room temperature and ends up as a gel, it cannot be reactivated for use.
L6274
Laminin from human placenta
liquid, BioReagent, suitable for cell culture
Laminin from human placenta
Synonym(s):
Cell culture grade laminin, Human Laminin
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Product Name
Laminin from human placenta, liquid, BioReagent, suitable for cell culture
biological source
human placenta
product line
BioReagent
form
liquid
concentration
0.5 mg/mL in Tris-buffered saline
technique(s)
cell culture | mammalian: suitable
surface coverage
1‑2 μg/cm2
UniProt accession no.
shipped in
dry ice
storage temp.
−70°C
Quality Level
Gene Information
human ... LAMB1(3912)
1 of 4
This Item | L4544 | L2020 | 11243217001 |
|---|---|---|---|
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable | technique(s) cell culture | mammalian: suitable |
| biological source human placenta | biological source human fibroblasts | biological source mouse (Engelbreth-Holm-Swarm mouse sarcoma basement membrane) | biological source mouse (Engelbreth-Holm-Swarm (EHS) sarcoma) |
| concentration 0.5 mg/mL in Tris-buffered saline | concentration - | concentration 1-2 mg/mL in Tris-buffered saline | concentration - |
| form liquid | form liquid | form aqueous solution | form solution |
| storage temp. −70°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| Gene Information human ... LAMB1(3912) | Gene Information human ... LAMB1(3912) | Gene Information mouse ... Lama1(16772), Lamb1(16777), Lamc1(226519), Lamc2(16782) | Gene Information mouse ... Lamb2(16779) |
Application
Laminin supports growth and differentiation of many cell types including epithelial, endothelial, neural, muscle and liver cells. It is recommended for use as a cell culture substratum at 1-2 μg/cm2. The optimal concentration does depend on cell type as well as the application or research objectives.
Biochem/physiol Actions
Laminin proteins are integral components of structural scaffolding in animal tissues. They associate with type IV collagen via entactin and perlecan and bind to cell membranes through integrin receptors, dystroglycan glycoprotein complexes and Lutheran blood group glycoproteins. Laminin has active domains for collagen binding, cell adhesion, heparin binding, and neurite outgrowth fragment.
Disclaimer
It is recommended to store this product at -70°C, where it will remain stable for two years.
Other Notes
Laminin is an extracellular matrix multidomain trimeric glycoprotein, and is the main non-collagenous component of basal lamina that supports adhesion, proliferation and differentiation. Laminin is composed of both A, B1 and B2 chains, which are connected by many disulfide bonds. This laminin product is purified from human placenta using pepsin treatment and immunoaffinity chromatography. SDS-PAGE results in two major bands at 50 kDa and 130-160 kDa.
Preparation Note
This product is supplied at a concentration of 0.5 mg/mL in 50 mM Tris-HCl, with 150 mM NaCl. It is 0.2 μm filtered. Thaw this solution slowly before use at 2-8°C to avoid gel formation. For use as a coating, dilute in a sterile balanced salt solution, coat culture surface with a minimal volume and incubate at 37°C for two hours. Wash 3 times with PBS before plating cells. Laminin coatings can be stored for one month at 2-8°C.
Storage Class
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Satu Marja Myllymäki et al.
PloS one, 6(5), e19453-e19453 (2011-05-17)
Formation of apical compartments underlies the morphogenesis of most epithelial organs during development. The extracellular matrix (ECM), particularly the basement membrane (BM), plays an important role in orienting the apico-basal polarity and thereby the positioning of apical lumens. Integrins have
Danielle K Lewis et al.
Aging cell, 7(6), 836-849 (2008-09-10)
Astrocytes comprise a large proportion of the central nervous system support cells and play a critical role in neural injury and repair. The present study examined the impact of ovarian aging using an ex vivo model system, where astrocytes were
Terhi P Teräväinen et al.
PloS one, 8(8), e71485-e71485 (2013-08-27)
The properties of epithelial cells within tissues are regulated by their immediate microenvironment, which consists of neighboring cells and the extracellular matrix (ECM). Integrin heterodimers orchestrate dynamic assembly and disassembly of cell-ECM connections and thereby convey biochemical and mechanical information
Kelly E Fathers et al.
The American journal of pathology, 176(1), 446-460 (2009-12-17)
The v-Crk protein was originally isolated as the oncogene fusion product of the CT10 chicken retrovirus. Cellular homologues of v-Crk include Crk, which encodes two alternatively spliced proteins (CrkI and CrkII), and CrkL. Though CrkI/II proteins are elevated in several
Chiyi Xiong et al.
Scientific reports, 10(1), 520-520 (2020-01-18)
The tyrosine kinase receptor EphB4 is frequently overexpressed in ovarian and other solid tumors and is involved in interactions between tumor cells and the tumor microenvironment, contributing to metastasis. Trans-interaction between EphB4 and its membrane-bound ligand ephrin B2 (EFNB2) mediates
Articles
Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.
Protocols
Coating surfaces with laminin for culturing cells requires specific conditions for optimal results. Protocols for coating coverslips to culture neurospheres and general cell culture are included.
Related Content
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How do you prepare Product L6274, Laminin from human placenta?
1 answer-
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How long can cover slips coated with Product L6274, Laminin from human placenta, be stored?
1 answer-
Laminin coatings, such as laminin coated cover slips last about one month at 2-8°C. Do not use the product if discoloration or spider web formations appear on the surface of the coated material.
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What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Is Product L6274, Laminin from human placenta, laminin 1 or 2?
1 answer-
Fifteen laminin trimers have been identified. We do not determine what type of laminin this is. Typically laminin from placenta is considered laminin-2/4 and laminin from Engelbreth-Holm-Swarm murine sarcoma basement membrane is laminin-1.
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