T1775

L-Thyroxine

powder, BioReagent, suitable for cell culture

• Synonym(s): 3,3′,5,5″-Tetraiodo-L-thyronine, 3-[4-(4-Hydroxy-3,5-diiodophenoxy)-3,5-diiodophenyl]-L-alanine, T₄
• Linear Formula: HOC₆H₂(I)₂OC₆H₂(I)₂CH₂CH(NH₂)CO₂H
• CAS Number: 51-48-9
• Molecular Weight: 776.87
• NACRES: NA.77
• PubChem Substance ID: 24899990
• eCl@ss: 32190102
• UNSPSC Code: 12352209
• EC Number: 200-101-1
• MDL number: MFCD00002595
• Beilstein/REAXYS Number: 2228515
• Biological source: synthetic
• Form: powder
• Assay: ≥98% (HPLC)

Properties

• Product Name: L-Thyroxine, powder, BioReagent, suitable for cell culture
• InChI key: XUIIKFGFIJCVMT-LBPRGKRZSA-N
• InChI: 1S/C15H11I4NO4/c16-8-4-7(5-9(17)13(8)21)24-14-10(18)1-6(2-11(14)19)3-12(20)15(22)23/h1-2,4-5,12,21H,3,20H2,(H,22,23)/t12-/m0/s1
• SMILES string: N[C@@H](Cc1cc(I)c(Oc2cc(I)c(O)c(I)c2)c(I)c1)C(O)=O
• biological source: synthetic
• product line: BioReagent
• assay: ≥98% (HPLC)
• form: powder
• potency: 5-50 ng/mL
• technique(s): cell culture | mammalian: suitable
• mp: 223 °C (dec.) (lit.)
• solubility: 4 M NH₄OH in methanol: 50 mg/mL, clear to hazy, yellow
• shipped in: ambient
• storage temp.: room temp
• Quality Level: 200
• Gene Information: human ... THRA(7067), THRB(7068), TTR(7276) ; mouse ... Cnr2(12802) ; rat ... Cnr1(25248)

Description

Application

L-Thyroxine has been used to investigate its role in colorectal cancer cell lines HCT 116 (APC wild type) and HT-29 (APC mutant).[1] It has also been used to evaluate its effects on pro-inflammatory tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), angiogenic vascular endothelial growth factor (VEGF), leukocyte adhesion, intercellular adhesion molecule 1 (ICAM-1) and E-selectin in human umbilical vein endothelial cells (HUVECs).[2]

Biochem/physiol Actions

L-Thyroxine (T₄) and triiodo-L-thyronine (T₃) are iodine-containing hormones produced from thyroglobulin in the thyroid follicular cells. The stimulation of metabolic rate and regulation of growth and development by these hormones appear to be due to their effects on DNA transcription and, thus, protein synthesis.

General description

Thyroxine (T₄) is a tetraiodo derivative of the p-hydroxyphenyl ether of tyrosine,[3] which functions as growth factors or cell growth inhibitors.[4] T₄ is used to treat hypothyroidism[5] and thyroid carcinoma.[6] It regulates energy metabolism.[7]

Preparation Note

For long term storage, keep stock solutions at -20° in working aliquots and avoid repeated freeze/thaw cycles. Solutions stored at 2-8 °C are stable for up to 30 days.

To prepare 20 μg/ml stock solution, dissolve in 50 ml sterile culture medium per mg thyroxine.

Safety Information

• pictograms: GHS08
• signalword: Danger
• hcodes: H372
• pcodes: P260 - P264 - P270 - P314 - P501
• Hazard Classifications: STOT RE 1
• target_organs: Thyroid,Cardio-vascular system,Kidney
• Storage Class: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)