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The serum complement system: a simplified laboratory exercise to measure the activity of an important component of the immune system.

Advances in physiology education (2008-12-03)
Jordan E Inglis, Kimberly A Radziwon, Gregory D Maniero
ABSTRACT

The immune system is a vital physiological component that affords animals protection from disease and is composed of innate and adaptive mechanisms that rely on cellular and dissolved components. The serum complement system is a series of dissolved proteins that protect against a variety of pathogens. The activity of complement in serum can be determined by its ability to lyse red blood cells in vitro. Here, we describe a modification of a standard complement hemolysis assay that makes an interesting and informative laboratory exercise suitable for a variety of courses including physiology.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Complement sera human, lyophilized powder
Sigma-Aldrich
Complement Serum Standard human, aqueous solution