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Interaction of choline salts with artificial biological membranes: DSC studies elucidating cellular interactions.

Biochimica et biophysica acta (2013-04-02)
Katherine D Weaver, Matthew P Van Vorst, R Vijayaraghavan, Douglas R Macfarlane, Gloria D Elliott
ABSTRACT

To better understand the relationship between the relative cytotoxicity of diluted ionic liquids and their specific interaction with biological membranes, the thermotropic behavior of model lipid membrane systems formulated in a series of choline based organic salts was investigated. Unilamellar vesicles prepared from dipalmitoylphosphatidylcholine were exposed to a series of choline phosphate salts at a concentration of 10mM at pH7.40, and the gel to liquid-crystalline state transition was examined using differential scanning calorimetry. The choline salts that were observed to have a low relative toxicity in previous studies induced minimal changes in the lipid phase transition behavior of these model membranes. In contrast, the salts choline bis(2,4,4-trimethylpentyl)phosphinate and choline bis(2-ethylhexyl)phosphate, both of which were observed to have high relative toxicity, caused distinct disruptions in the lipid phase transition behavior, consistent with penetration of the salts into the acyl chains of the phospholipids. choline bis(2,4,4-trimethylpentyl)phosphinate reduced the Tm and enthalpy of the main transition of dipalmitoylphosphatidylcholine while choline bis(2-ethylhexyl)phosphate induced the equilibration of alternate phases.

MATERIALS
Product Number
Brand
Product Description

Sigma-Aldrich
Choline chloride, ≥99%
Sigma-Aldrich
Choline chloride, ≥98%
Sigma-Aldrich
Choline chloride, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥98%
Sigma-Aldrich
Choline chloride, BioUltra, ≥99.0% (AT)
Supelco
Choline chloride, Pharmaceutical Secondary Standard; Certified Reference Material