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Merck

17-10078

EZ-Magna ChIP® HT96 Chromatin Immunoprecipitation Kit

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NACRES:
NA.84
UNSPSC Code:
41105331

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technique(s)

immunoprecipitation (IP): suitable

Quality Level

100

Application

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Features and Benefits

  • Complete set of materials for up to 96 ChIP reactions
  • Protein A+G bead blend for ChIP with a broader range of antibodies than A or G alone
  • Low Chromatin requirements: 10, 000 to 100, 000 cells per reaction
  • Includes negative and positive control antibodies and control primer set to simplify validation of experimental procedure
  • Optimized streamlined protocol with only a single buffer for sonication, IP, or wash; and protocols for automated liquid handling systemsProtocols for using cells or tissues
  • Direct analysis of DNA without additional clean-up steps
  • Compatible with ChIPAb+ validated antibody and primer sets

General description

The EZ-Magna ChIP HT96 kit allows the performance of chromatin Immunoprecipitation in a 96-well plate-based format. This reliable streamlined approach works for both cells and tissues and can be performed using either a multi-channel pipette or an automated liquid handling instrument. Each kit includes a complete set of validated, quality controlled reagents, positive and negative control antibodies and validated primer set plus a detailed protocol.

Other Notes

Magna ChIP protein A/G magnetic beads;HT96 Nuclei Isolation Buffer ;HT96 ChIP Buffer (Sonication/ChIP/Wash);Low Stringency IP Wash Buffer ;HT96 ChIP Elution Buffer ;Proteinase K Solution;Protease Inhibitor Cocktail III;10X Glycine ;10X PBS ;96 Well ChIP Plate ;96 Well Thermal Plate;Plate Seal;Strip Caps;Anti-Trimethyl-Histone H3 (Lys4) -positive control antibody;Normal Rabbit IgG -negative control antibody;GAPDH Primers - PCR positive control

Packaging

Kit capacity: 96 chromatin immunoprecipitation assays

Legal Information

MAGNA CHIP is a registered trademark of Merck KGaA, Darmstadt, Germany

Certificados de análisis (COA)

Busque Certificados de análisis (COA) introduciendo el número de lote del producto. Los números de lote se encuentran en la etiqueta del producto después de las palabras «Lot» o «Batch»

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Magna ChIP™ protein A/G bead blends simplify the ChIP process and reduce background signals

Chromatin immunoprecipitation (ChIP) has been widely adapted for the study of gene-specific and genome-wide distribution of specific DNA- and RNA-binding proteins or protein modifications. Similar to standard protein immunoprecipitation assays, ChIP involves isolation of immunocomplexes using a solid medium, such as agarose or magnetic beads, coupled to either IgG binding recombinant protein A or protein G. In a typical ChIP experiment either protein A or G is selected for enrichment depending on the antibody isotype. However, proteins A and G possess differing affinities for human and mouse IgGs. Complicating this choice, for some antibody isotypes there is affinity for both protein A and G. In addition, we have observed that independent of the isotype the affinity of a specific antibody for protein A or G can vary depending on the specific clone, purification method, and source.

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Superior enrichment, low background. With performance proven for both qPCR and ChIP-seq analysis, the Magna ChIP™ HiSens kit may be the only ChIP kit you’ll ever need. Outperforming any competing kit, this revolutionary approach to ChIP enables enrichment from both low and high amounts of input chromatin while also delivering low backgrounds and high signal-to-noise ratios for ultra-sensitive detection.

An Introduction to Antibodies and Their Applications

The 3rd edition of An Introduction to Antibodies and Their Applications provides a concise overview of some of the key features for the use of antibodies and immunochemical techniques in biological research. This handy reference guide supplements the techniques described in literature, recorded in general laboratory procedures, and described on individual product data sheets. Antibody design, development, and production are our expertise. Stringent validation of our antibodies is only one component of a comprehensive process we undertake to provide the antibodies most cited by the research community (see section Antibody Quality on page 2 for an in-depth look at our expertise).

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